Calnexin-CT Antibody | StressMarq Biosciences SPC-182B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Calnexin-CT Antibody

catalog :

SPC-182B

quantity :

200 µg

price :

346.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SPC-182B

Product Name :

Calnexin-CT Antibody

Description :

Rabbit Anti-Dog Calnexin-CT Polyclonal

Target :

Calnexin

Conjugate :

Unconjugated

2021 List Price :

346.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Tags and Cell Markers Organelle Markers

Alternative Name(s) :

Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody

Size :

200 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Dog Calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.

Immunogen Species :

Dog

Accession Number :

NP_001003232.1

Swiss-Prot :

P24643

Applications :

WB IHC ICC/IF IP FCM

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Mk Ck Dg GP Hm Pg Qu Rb Sh Dr Xe

Species Reactivity Full Name :

Human Mouse Rat Bovine Monkey Chicken Dog Guinea Pig (Cavia porcellus) Hamster Pig Avian Quail Rabbit Sheep Fruit Fly (Drosophila melanogaster) African clawed frog (Xenopus laevis)

Antibody Dilution :

WB (1:2000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS pH7.2, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects the C-terminal domain of Calnexin ~90kDa. Weak detection in Chicken, Drosophila, and Xenopus tissues.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-182B, RRID: AB_2068990

Certificate of Analysis :

A 1:2000 dilution of SPC-182 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Endoplasmic Reticulum Endoplasmic Reticulum Membrane Melanosome

Scientific Background :

Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

References :

1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science. 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry. 33: 3229. 3. Schrag J. et al. (2001) Molecular Cell. 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-182_Calnexin-CT_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite2.png SPC-182_Calnexin-CT_Antibody_WB_Rat_tissue-mix_1.png SPC-182_Calnexin-CT_Antibody_IHC_Mouse_backskin_1.png SPC-182_Calnexin-CT_Antibody_ICC-IF_Human_HaCaT-cells_2.png SPC-182_Calnexin-CT_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-Calnexin-CT Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-182) Rabbit Anti-Calnexin-CT Antibody used in Western blot (WB) on Rat tissue mix (SPC-182) Rabbit Anti-Calnexin-CT Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-182) Rabbit Anti-Calnexin-CT Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-182) Rabbit Anti-Calnexin-CT Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-182)

more info or order :

StressMarq Biosciences product webpage