HSP22 Antibody | StressMarq Biosciences SPC-181D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP22 Antibody

catalog :

SPC-181D

quantity :

100 µl

price :

251.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SPC-181D

Product Name :

HSP22 Antibody

Description :

Rabbit Anti-Human HSP22 Polyclonal

Target :

HSP22

Conjugate :

Unconjugated

2021 List Price :

251.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Neuroscience Neurodegeneration

Alternative Name(s) :

Alpha crystallin C chain Antibody, CMT2L Antibody, CRYAC Antibody, DHMN2 Antibody, H11 Antibody, Heat shock 22kDa protein 8 Antibody, HMN2 Antibody, HSB8 Antibody, HSPB8 Antibody, HSP22 Antibody

Size :

100 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Human HSP22

Immunogen Species :

Human

Accession Number :

NP_055180.1

Swiss-Prot :

Q9UJY1

Applications :

WB IHC ICC/IF IP

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), ICC/IF (1:100), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~22kDa. Does not cross-react with HSP27 or alpha-crystallin.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-181D, RRID: AB_2121090

Certificate of Analysis :

A 1:1000 dilution of SPC-181 was sufficient for detection of HSP22 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

HSP22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to HSP27. Similar to most other sHSPs, HSP22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). HSP22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

References :

1. Kappe G., et al. (2001) Biochem Biophys Acta. 1520: 1-6. 2. Benndorf R., et al. (2001) J Biol Chem. 276: 26753-26761. 3. Sun X., et al. (2004) J Biol Chem. 279: 2394-2402. 4. Kim M.V., et al. (2004) Biochem Biophys Res Commun. 325: 649-652. 5. Wilhelmus M.M., et al. (2006) Acta Neuropathol. (Berl) 111: 139-149.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-181_HSP22_Antibody_ICC-IF_Human_HaCaT-cells_3.png SPC-181_HSP22_Antibody_WB_Rat_Skeletal-muscle-lysates_1.png SPC-181_HSP22_Antibody_IHC_Mouse_backskin_3.png SPC-181_HSP22_Antibody_IHC_Mouse_Spinal-cord_1.png

Image Alternative Text :

Rabbit Anti-HSP22 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-181) Rabbit Anti-HSP22 Antibody used in Western blot (WB) on Rat Skeletal muscle lysates (SPC-181) Rabbit Anti-HSP22 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-181) Rabbit Anti-HSP22 Antibody used in Immunohistochemistry (IHC) on Mouse Spinal cord (SPC-181)

more info or order :

StressMarq Biosciences product webpage