HSP90 beta Antibody | StressMarq Biosciences SPC-177D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP90 beta Antibody

catalog :

SPC-177D

quantity :

100 µl

price :

251.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SPC-177D

Product Name :

HSP90 beta Antibody

Description :

Rabbit Anti-Human HSP90 beta Polyclonal

Target :

HSP90 beta

Conjugate :

Unconjugated

2021 List Price :

251.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers

Alternative Name(s) :

HSP84 Antibody, HSP90 Antibody, HSP86 Antibody, HSP89 Antibody, HSP90B Antibody, HSP90BETA Antibody, HSP90N Antibody, HSPC2 Antibody, HSPCA Antibody, HSPCAL1 Antibody, HSPCB Antibody, HSPN Antibody, LAP2 Antibody, NY REN 38 antigen Antibody

Size :

100 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Full length protein HSP90

Immunogen Species :

Human

Accession Number :

NP_031381.2

Swiss-Prot :

P08238

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~90kda. Does not cross-react with HSP90α.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-177D, RRID: AB_2279948

Certificate of Analysis :

A 1:1000 dilution of SPC-177 was sufficient for detection of HSP90 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Cytoplasm Melanosome

Scientific Background :

HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5. When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

References :

1. Arlander S.J.H., et al. (2003) J Biol Chem. 278: 52572-52577. 2. Pearl H., et al. (2001) Adv Protein Chem. 59:157-186. 3. Neckers L., et al. (2002) Trends Mol Med. 8:S55-S61. 4. Pratt W., Toft D. (2003) Exp Biol Med. 228:111-133. 5. Pratt W., Toft D. (1997) Endocr Rev. 18: 306–360. 6. Pratt W.B. (1998) Proc Soc Exptl Biol Med. 217: 420–434. 7. Whitesell L., et al. (1994) Proc Natl Acad Sci USA. 91: 8324–8328.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-177_Hsp90-beta_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-177_HSP90-beta_Antibody_WB_Human_Cell-line-lysates_1.png SPC-177_HSP90-beta_Antibody_IHC_Mouse_backskin_1.png SPC-177_Hsp90-beta_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png SPC-177_HSP90-beta_Antibody_WB_Human_Cell-line-lysates_2.png

Image Alternative Text :

Rabbit Anti-Hsp90 beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-177) Rabbit Anti-HSP90 beta Antibody used in Western blot (WB) on Human Cell line lysates (SPC-177) Rabbit Anti-HSP90 beta Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-177) Rabbit Anti-Hsp90 beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-177) Rabbit Anti-HSP90 beta Antibody used in Western blot (WB) on Human Cell line lysates (SPC-177)

more info or order :

StressMarq Biosciences product webpage