RAS Antibody | StressMarq Biosciences SPC-173D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

RAS Antibody

catalog :

SPC-173D

quantity :

100 µg

price :

257.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SPC-173D

Product Name :

RAS Antibody

Description :

Rabbit Anti-Human RAS Polyclonal

Target :

RAS

Conjugate :

Unconjugated

2021 List Price :

257.00 USD

Currency :

USD

Research Area(s) :

Cancer Cell Signaling Epigenetics and Nuclear Signaling

Alternative Name(s) :

GTPase Hras Antibody, GTPase Kras Antibody, GTPase Nras Antibody, Ha Ras Antibody, K Ras Antibody, K ras p21 Antibody, KRAS Antibody, NRAS Antibody, p21ras Antibody, RASH2 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)

Immunogen Species :

Human

Accession Number :

NP_035067.2

Swiss-Prot :

B2KGV5

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv

Species Reactivity Full Name :

Human Mouse Rat Bovine

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~21kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-173D, RRID: AB_2703838

Certificate of Analysis :

1 µg/ml of SPC-173 was sufficient for detection of Ras in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cell Membrane Golgi Apparatus Golgi Apparatus Membrane

Scientific Background :

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

References :

1. Boguski M.S. and McCormick F. (1993) Nature 366: 643–654. 2. Avruch J., et al. (1994) Trends Biochem. Sci.19: 279–283. 3. Buday L. and Downward J. (1993) Cell 73: 611–620. 4. Huang D.C., et al. (1993) Mol. Cell Biol. 13: 2420–2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682–4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114–131.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-173_RAS_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-173_RAS_Antibody_IHC_Mouse_backskin_1.png SPC-173_RAS_Antibody_WB_Mouse_Brain-Cortex-cell-lysates_1.png SPC-173_RAS_Antibody_ICC-IF_Human_HaCaT-cells_1.png SPC-173_RAS_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-173) Rabbit Anti-RAS Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-173) Rabbit Anti-RAS Antibody used in Western blot (WB) on Brain Cortex cell lysates (SPC-173) Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-173) Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-173)

more info or order :

StressMarq Biosciences product webpage