p38 Antibody | StressMarq Biosciences SPC-172D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

p38 Antibody

catalog :

SPC-172D

quantity :

100 µl

price :

209.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 1

Reference
Ta H, Le T, Ishii H, Takarada Iemata M, Hattori T, Hashida K, et al. Atf6? deficiency suppresses microglial activation and ameliorates pathology of experimental autoimmune encephalomyelitis. J Neurochem. 2016;139:1124-1137 pubmed publisher

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.

product information

Catalog No :

SPC-172D

Product Name :

p38 Antibody

Description :

Rabbit Anti-Human p38 Polyclonal

Target :

p38

Conjugate :

Unconjugated

2021 List Price :

209.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation Cancer

Alternative Name(s) :

CSAID Binding protein 1 Antibody, CSBP1 Antibody, CSBP2 Antibody, EXIP Antibody, MAP kinase MXI2 Antibody, MAPkinase p38alpha Antibody, MAPK14 Antibody, p38 ALPHA Antibody, p38 MAP kinase Antibody, p38 mitogen activated protein kinase Antibody, RK Antibody, SAPK 2A Antibody, Stress activated protein kinase 2A Antibody

Size :

100 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH

Immunogen Species :

Human

Accession Number :

NP_001306.1

Swiss-Prot :

Q16539

Applications :

WB IHC ICC/IF IP

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Mk Rb Pg Dg Hm Ck Sh GP

Species Reactivity Full Name :

Human Mouse Rat Bovine Monkey Rabbit Pig Dog Hamster Chicken Sheep Guinea Pig (Cavia porcellus)

Antibody Dilution :

WB (1:1000), ICC/IF (1:100), IP (1:250); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~43kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-172D, RRID: AB_1055444

Certificate of Analysis :

A 1:1000 dilution of SPC-172 was sufficient for detection of p38 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).

References :

1. Pearson G., et al (2001) Endocrine Reviews 22 (2): 153-183. 2. Fan Y., et al (2007) Mol. Cells 23 (1): 30-38. 3. Han J., et al. (1994) Science 265: 808-811. 4. Van L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell 115: 61-70. 6. Salojin K.V., et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S., et al. (2006) J Pharmacol Exp Ther. 318(1): 99-107.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-172_p38_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-172_p38_Antibody_WB_Human_cancer-cell-lines_1.png SPC-172_p38_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-172) Rabbit Anti-p38 Antibody used in Western blot (WB) on Human cancer cell lines (SPC-172) Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-172)

more info or order :

StressMarq Biosciences product webpage