antibody

Rab5 Antibody

SPC-168D

100 µg

240.00 USD

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat, bovine

western blot, immunohistochemistry, immunocytochemistry

Rab5 Antibody

Rabbit Anti-Human Rab5 Polyclonal

Rab5

Unconjugated

240.00 USD

USD

Cell Signaling Tags and Cell Markers Organelle Markers Protein Trafficking Vesicle Transport Neuroscience Cell Markers Neuron Markers Presynaptic Markers

Rab 5A Antibody, RAS associated protein RAB5A Antibody, Ras related protein Rab 5 A Antibody

100 µg

Antibodies

Polyclonal

Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues

Human

NP_004153.2

P20339

WB IHC ICC/IF

Rabbit

Hu Ms Rt Bv Mk

Human Mouse Rat Bovine Monkey

WB (1:1000), IHC (1:100), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.

Protein A Purified

PBS, 50% glycerol, 0.09% sodium azide

1 mg/ml

Detects ~26kDa.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-168D, RRID: AB_2177680

1 µl/ml of SPC-168 was sufficient for detection of Rab5 in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

Cell Membrane Endosome Early Endosome Membrane Melanosome

Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913 6. Woodman P.G. (2000) Traffic. 1: 695-701.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-168_Rab5_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-168_Rab5_Antibody_WB_Human_Cell-line-lysates_1.png SPC-168_Rab5_Antibody_IHC_Mouse_backskin_1.png SPC-168_Rab5_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Rabbit Anti-Rab5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-168) Rabbit Anti-Rab5 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-168) Rabbit Anti-Rab5 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-168) Rabbit Anti-Rab5 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-168)