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company name :
StressMarq Biosciences
product type :
antibody
product name :
PUMA (CT) Antibody
catalog :
SPC-166D
quantity :
100 µg
price :
300.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SPC-166D
Product Name :
PUMA (CT) Antibody
Description :
Rabbit Anti-Human PUMA (CT) Polyclonal
Target :
PUMA
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Cancer Apoptosis Intracellular Bcl2 Family Cancer Invasion/microenvironment Cell Signaling Metabolism Mitochondrial Metabolism
Alternative Name(s) :
BBC3 Antibody, BCL2 binding component 3 Antibody, p53 up regulated modulator of apoptosis Antibody, PUMA/JFY1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
C-terminal amino acids of human PUMA
Immunogen Species :
Human
Accession Number :
NP_001120712.1
Swiss-Prot :
Q9BXH3
Applications :
WB ICC/IF
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Ck
Species Reactivity Full Name :
Human Mouse Chicken
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS, 0.02% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~23kDa. Detects ~16kDa bands sometimes, possibly corresponding to PUMAβ.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-166D, RRID: AB_2064543
Certificate of Analysis :
1 µg/ml of SPC-166 was sufficient for detection of PUMA in 20 µg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Mitochondrion
Scientific Background :
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
References :
1. Nakano K., Vousden K.H. (2001) Mol Cell. 2001; 7(3): 683-94. 2. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. (2001) Mol Cell. 7(3): 673-82. 3. Han J., et al. (2001) Proc Natl Acad Sci U S A. 98(20): 11318-23.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-166_PUMA-CT_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-166_PUMA_Antibody_WB_Human_Mouse_K562-and-3T3-cell-lysates_1.png SPC-166_PUMA-CT_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SPC-166_PUMA_Antibody_ICC-IF_Human_K562-cells_1.png
Image Alternative Text :
Rabbit Anti-PUMA (CT) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-166) Rabbit Anti-PUMA Antibody used in Western blot (WB) on Human, Mouse K562 and 3T3 cell lysates (SPC-166) Rabbit Anti-PUMA (CT) Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-166) Rabbit Anti-PUMA Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human K562 cells (SPC-166)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.