BIM/BOD (IN2) Antibody | StressMarq Biosciences SPC-164D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

BIM/BOD (IN2) Antibody

catalog :

SPC-164D

quantity :

100 µg

price :

300.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Human Lung carcinoma epithelial cell line (A549) lysate showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-164). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-164) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

product information

Catalog No :

SPC-164D

Product Name :

BIM/BOD (IN2) Antibody

Description :

Rabbit Anti-Human BIM/BOD (IN2) Polyclonal

Target :

BIM

Conjugate :

Unconjugated

2021 List Price :

300.00 USD

Currency :

USD

Research Area(s) :

Cancer Apoptosis Intracellular Neuroscience Neurodegeneration Alzheimer's Disease Immunology Innate Immunity Macrophage Cell Signaling Protein Phosphorylation Serine / Threonine Kinases MAPK Pathway Cancer Invasion/microenvironment Cancer Tumor Biomarkers Oncoproteins Cell Signaling Metabolism Metabolism processes

Alternative Name(s) :

BCL2 like protein 11 Antibody, Bcl2 interacting mediator of cell death Antibody, Bcl2 interacting protein Bim Antibody, BCL@ like 11 Antibody, BAM Antibody, BimEl Antibody, BimL Antibody, BOD Antibody, BIM beta 6 Antibody, BIM Beta7 Antibody, BIM alpha 3 Antibody, BIM alpha4 Antibody, BIM alpha5 Antibody, BIM alpha6 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Internal central amino acids of human Bim

Immunogen Species :

Human

Accession Number :

NP_619527.1

Swiss-Prot :

O43521

Applications :

WB ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:400), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~23kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-164D, RRID: AB_2290429

Certificate of Analysis :

2.5 µg/ml of SPC-164 was sufficient for detection of Bim in 20 µg of K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Mitochondrion Endomembrane System

Scientific Background :

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).

References :

1. O'Connor L., Strasser A., O'Reilly L.A., et al. (1998) EMBO J. 17:384-395. 2. Hsu S.Y., Lin P., and Hsueh A.J. (1998) Mol. Endocrinol. 12:1432-40.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-164_BIM_Antibody_ICC-IF_Human_K562-cells_1.png SPC-164_BIM_Antibody_WB_Human_A549-cell-lysates_1.png

Image Alternative Text :

Rabbit Anti-BIM Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human K562 cells (SPC-164) Rabbit Anti-BIM Antibody used in Western blot (WB) on Human Lung carcinoma epithelial cell line (A549) lysate (SPC-164)

more info or order :

StressMarq Biosciences product webpage