Phosphotyrosine Antibody | StressMarq Biosciences SPC-161F product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Phosphotyrosine Antibody

catalog :

SPC-161F

quantity :

400 µl

price :

310.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Immunohistochemistry analysis using Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161). Tissue: breast cancer. Species: Human . Primary Antibody: Rabbit Anti-Phosphotyrosine Polyclonal Antibody (SPC-161) at 1:100.

product information

Catalog No :

SPC-161F

Product Name :

Phosphotyrosine Antibody

Description :

Rabbit Anti-Phosphotyrosine Polyclonal

Target :

Phosphotyrosine

Conjugate :

Unconjugated

2021 List Price :

310.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation

Alternative Name(s) :

Phospho-tyrosine Antibody

Size :

400 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Phosphotyrosine conjugated to KLH

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

ALL

Species Reactivity Full Name :

Species Independent

Antibody Dilution :

WB (1:500), IHC (1:100), ICC/IF (1:50), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.0, 50% glycerol, 0.01% sodium azide

Concentration :

0.25 mg/ml

Specificity :

Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-161F, RRID: AB_854256

Certificate of Analysis :

A 1:250 dilution of SPC-161 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.

Scientific Background :

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).

References :

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-163_Phosphotyrosine_Antibody_IHC_Hu
man_breast-cancer_1.png
SPC-161_Phosphotyrosine_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-161_Phosphotyrosine_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-161) Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-161) Rabbit Anti-Phosphotyrosine Antibody used in Immunohistochemistry (IHC) on Human breast cancer (SPC-161)

more info or order :

StressMarq Biosciences product webpage