product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Phosphothreonine Antibody
catalog :
SPC-154F
quantity :
400 µl
price :
310.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-154F image 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphothreonine Polyclonal Antibody (SPC-154) at 1:60 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Phosphothreonine Antibody. (C) Composite.
product information
Catalog No :
SPC-154F
Product Name :
Phosphothreonine Antibody
Description :
Rabbit Anti-Phosphothreonine Polyclonal
Target :
Phosphothreonine
Conjugate :
Unconjugated
2021 List Price :
310.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Post-translational Modifications Phosphorylation
Alternative Name(s) :
Phospho-threonine Antibody
Size :
400 µl
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Phosphothreonine conjugated to KLH
Applications :
WB ICC/IF IP ELISA
Host Species :
Rabbit
Species Reactivity Abbreviation :
ALL
Species Reactivity Full Name :
Species Independent
Antibody Dilution :
WB (1:500), ICC/IF (1:60), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.01% sodium azide
Concentration :
0.25 mg/ml
Specificity :
Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-154F, RRID: AB_854250
Certificate of Analysis :
2 µg/ml of SPC-154 was sufficient for detection of phosphorylation signal in western blot analysis using mouse spleen extract treated with Vanadium.
Scientific Background :
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
References :
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9(16): 2034-2041.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-154_Phosphothreonine_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png
SPC-154_Phosphothreonine_Antibody_WB_Mo
use_brain-cell-lysates_1.png
SPC-154_Phosphothreonine_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png
Image Alternative Text :
Rabbit Anti-Phosphothreonine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-154) Rabbit Anti-Phosphothreonine Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-154) Rabbit Anti-Phosphothreonine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-154)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.