product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Phosphoserine Antibody
catalog :
SPC-149F
quantity :
400 µl
price :
310.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
HRP
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SPC-149F image 1
Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149). Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody (SPC-149) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1µM okadaic acid).
product information
Catalog No :
SPC-149F
Product Name :
Phosphoserine Antibody
Description :
Rabbit Anti-Phosphoserine Polyclonal
Target :
Phosphoserine
Conjugate :
Unconjugated
2021 List Price :
310.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Post-translational Modifications Phosphorylation
Alternative Name(s) :
Phospho-ser Antibody, pS Antibody, pSer Antibody, Phospho-serine antibody
Size :
400 µl
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Phosphoserine conjugated to KLH, and phosvitin mixture
Applications :
WB IHC ICC/IF IP ELISA
Host Species :
Rabbit
Species Reactivity Abbreviation :
ALL
Species Reactivity Full Name :
Species Independent
Antibody Dilution :
WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS, 50% glycerol, 0.01% sodium azide
Concentration :
0.25 mg/ml
Specificity :
Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-149F, RRID: AB_854246
Certificate of Analysis :
2 µg/ml of SPC-149 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 µM okadaic acid.
Scientific Background :
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
References :
1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Ostrovsky P.C. (1995) Genes Dev. 9(16): 2034-2041.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-149_Phosphoserine_Antibody_WB_Mouse
_Spleen-lysates_1.png
SPC-149_Phosphoserine_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite2.png
Image Alternative Text :
Rabbit Anti-Phosphoserine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-149) Rabbit Anti-Phosphoserine Antibody used in Western blot (WB) on Mouse Spleen lysates (SPC-149)
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.