Akt2 (PKB beta) Antibody | StressMarq Biosciences SPC-148F product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Akt2 (PKB beta) Antibody

catalog :

SPC-148F

quantity :

400 µl

price :

263.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, ELISA, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of multiple Cell line lysates showing detection of AKT2 protein using Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-AKT2 Polyclonal Antibody (SPC-148) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

product information

Catalog No :

SPC-148F

Product Name :

Akt2 (PKB beta) Antibody

Description :

Rabbit Anti-Human Akt2 (PKB beta) Polyclonal

Target :

AKT2

Conjugate :

Unconjugated

2021 List Price :

263.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation

Alternative Name(s) :

AKT2 Antibody, PKB Antibody, PKB beta Antibody, PRKBB Antibody, protein kinase Akt2 Antibody, RAC BETA Antibody

Size :

400 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH

Immunogen Species :

Human

Accession Number :

NP_001617.1

Swiss-Prot :

P31751

Applications :

WB ICC/IF ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Ck Dg GP Hm Mk Pg Rb Sh Xe

Species Reactivity Full Name :

Human Mouse Rat Bovine Chicken Dog Guinea Pig (Cavia porcellus) Hamster Monkey Pig Rabbit Sheep African clawed frog (Xenopus laevis)

Antibody Dilution :

WB (1:1000), ICC/IF (1:25); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

TBS, 50% glycerol, 0.01% sodium azide

Concentration :

0.25 mg/ml

Specificity :

Detects ~65kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-148F, RRID: AB_2225163

Certificate of Analysis :

A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Cytoplasm Nucleus Cell Membrane

Scientific Background :

Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).

References :

1. Downward J. (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil D.P., and Hemmings B.A. (2001). Biochem Sci. 26(11):657-64. 3. Nicholson K.M., Anderson N.G. (2002). Cell Signal. 14(5): 381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-148_AKT2_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-148_AKT2_Antibody_WB_multiple_Cell-line-lysates_1.png

Image Alternative Text :

Rabbit Anti-AKT2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-148) Rabbit Anti-AKT2 Antibody used in Western blot (WB) on multiple Cell line lysates (SPC-148)

more info or order :

StressMarq Biosciences product webpage