p90 RSK1 Antibody | StressMarq Biosciences SPC-147F product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

p90 RSK1 Antibody

catalog :

SPC-147F

quantity :

400 µl

price :

184.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p90 RSK1 Polyclonal Antibody (SPC-147) at 1:50 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p90 RSK1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

product information

Catalog No :

SPC-147F

Product Name :

p90 RSK1 Antibody

Description :

Rabbit Anti-Human p90 RSK1 Polyclonal

Target :

RSK1

Conjugate :

Unconjugated

2021 List Price :

184.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation

Alternative Name(s) :

p90RSK Antibody, RSK1 Antibody, RPS6KA1 Antibody, p90 RSK1 Antibody, MAPKAPK-1a Antibody, p90S6K Antibody, Ribosomal S6 kinase 1 Antibody, RSK1 Antibody, MAP kinase-activated protein kinase 1a Antibody, MAPK-activated protein kinase 1a Antibody, EC:2.7.11.1 Antibody, p90-RSK 1 Antibody, Ribosomal protein S6 kinase alpha-1 Antibody, S6K-alpha-1 Antibody, p90RSK1 Antibody, 90 kDa ribosomal protein S6 kinase 1 Antibody, RSK-1 Antibody, MAPKAP kinase 1a Antibody, MAPKAPK1A Antibody

Size :

400 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Human p90 RSK1 C-terminal peptide, conjugated to KLH

Immunogen Species :

Human

Accession Number :

NP_002944.2

Swiss-Prot :

Q15418

Applications :

WB ICC/IF IP ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv

Species Reactivity Full Name :

Human Mouse Rat Bovine

Antibody Dilution :

WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS, 50% glycerol, 0.01% sodium azide

Concentration :

0.25 mg/ml

Specificity :

Detects ~90kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-147F, RRID: AB_2253959

Certificate of Analysis :

0.25 mg/ml was sufficient for detection of SPC-147 in lysates prepared from mouse brain, spleen and intestine.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

The p90 ribosomal S6 kinase (Rsk) family comprises four mammalian serine/threonine kinases (Rsk1–4). In the past, S6K1 and S6K2 were thought to be the predominant operating S6 kinases, however RSK 1 and 2 have been shown to phosphorylate S6 in response to the ERK pathway, or otherwise known as the extra cellular signal-regulated kinases pathway. RSK 1 in particular is very multifunctional as it participates in nuclear signaling, regulates nuclear factors, regulates several transcription factors like c-Fos, and interacts with the transcriptional coactivator CREB-binding protein. As a result, RSK1 seems to have an important role in cellular growth control and proliferation. (1-5).

References :

1. Roux, P. (2007). UCSD-Nature Molecule Pages. 2. Fro¨din, M. and Gammeltoft, S. (1999) Mol. Cell. Endocrinol. 151: 65-77. 3. Merienne, K. et al. (1998) J. Med. Genet. 35: 890-894. 4. De Cesare, D. et al. (1998) Proc. Natl. Acad. Sci. 95: 12202-12207. 5. Joel, P. B. et al. (1998) Mol. Cell. Biol. 18: 1978-1984.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-147_p90-RSK1_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-147_RSK1_Antibody_WB_Mouse_brain-cell-lysates_1.png SPC-147_p90-RSK1_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-p90 RSK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-147) Rabbit Anti-RSK1 Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-147) Rabbit Anti-p90 RSK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-147)

more info or order :

StressMarq Biosciences product webpage