p70 S6K Antibody | StressMarq Biosciences SPC-146F product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

p70 S6K Antibody

catalog :

SPC-146F

quantity :

400 µl

price :

184.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p70 S6K Polyclonal Antibody (SPC-146) at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Perinuclear region of cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p70 S6K Antibody. (C) Composite.

product information

Catalog No :

SPC-146F

Product Name :

p70 S6K Antibody

Description :

Rabbit Anti-Human p70 S6K Polyclonal

Target :

p70 S6K

Conjugate :

Unconjugated

2021 List Price :

184.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Phosphorylation

Alternative Name(s) :

p70 S6 kinase beta (phospho T229) Antibody, p70-beta Antibody, p70S6Kb Antibody, S6K beta Antibody, S6K2 Antibody, SRK Antibody, STK14B Antibody

Size :

400 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Human p70 S6K c-terminal peptide –KLH conjugates

Immunogen Species :

Human

Accession Number :

NP_003943.2

Swiss-Prot :

Q9UBS0

Applications :

WB ICC/IF IP ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv

Species Reactivity Full Name :

Human Mouse Rat Bovine

Antibody Dilution :

WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

TBS, 50% glycerol, 0.01% sodium azide

Concentration :

0.25 mg/ml

Specificity :

Detects ~70kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-146F, RRID: AB_2285379

Certificate of Analysis :

0.25 mg/ml was sufficient for detection of SPC-146 in lysates prepared from mouse muscle and mouse brain.

Cellular Localization :

Cytoplasm Nucleus Cell Junction Mitochondrion Mitochondrion Outer Membrane

Scientific Background :

Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/ dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and C-terminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.

References :

1. Gout, I. et al. (1998). J Biol Chem. 273 (46):30061-30064. 2. Val'Ovka, TI. et al. (2000). Ukr Biokhim Zh. 72(3):31-7. 3. Kawasome, H. et al. (1998) Proc. Natl. Acad.Sci. 95 4. Montagne, J. et al. 1999) Science 285 5. Shima, H. et al. (1998) EMBO J 17: 6649-6659. 6. Lee-Fruman, K. et al. (1999) Oncogene 18: 5108- 5114.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-146_p70-S6K_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-146_p70-S6K_Antibody_WB_Mouse_brain-cell-lysates_1.png SPC-146_p70-S6K_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-p70 S6K Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-146) Rabbit Anti-p70 S6K Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-146) Rabbit Anti-p70 S6K Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-146)

more info or order :

StressMarq Biosciences product webpage