p33 ING1 Antibody | StressMarq Biosciences SPC-145F product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

p33 ING1 Antibody

catalog :

SPC-145F

quantity :

400 µl

price :

289.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p33 ING1 Polyclonal Antibody (SPC-145). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p33 ING1 Polyclonal Antibody (SPC-145) at 1:50 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.

product information

Catalog No :

SPC-145F

Product Name :

p33 ING1 Antibody

Description :

Rabbit Anti-Human p33 ING1 Polyclonal

Target :

ING1

Conjugate :

Unconjugated

2021 List Price :

289.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Epigenetics and Nuclear Signaling Cancer

Alternative Name(s) :

Growth inhibitor ING1 Antibody, ING1 Antibody, inhibitor of growth 1 Antibody, p24ING1c Antibody, p33 Antibody, p33ING Antibody, p33ING1b Antibody, p33ING1c Antibody, p47 Antibody, p47ING1a Antibody, tumor suppressor ING1 Antibody

Size :

400 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Mouse p33 ING1 N-terminal peptide –KLH conjugates

Immunogen Species :

Human

Accession Number :

NP_036049.2

Swiss-Prot :

Q9QXV3

Applications :

WB ICC/IF ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms

Species Reactivity Full Name :

Human Mouse

Antibody Dilution :

WB (1:1000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

TBS, 50% glycerol, 0.01% sodium azide

Concentration :

0.25 mg/ml

Specificity :

Detects ~33kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-145F, RRID: AB_2127644

Certificate of Analysis :

0.25 mg/ml was sufficient for detection of SPC-145 in lysates prepared from human melanoma cell lines by western blot analysis.

Cellular Localization :

Nucleus

Scientific Background :

The p33ING1 protein encodes a 33-kD, 294-amino acid protein that displays the characteristics of a tumor suppressor gene. The p33ING1 protein is a regulator of cell cycle, senescence, and apoptosis. It can mediate growth arrest, anchor dependent growth and influence chemo sensitivity (1-4). It also has a proposed role as a growth regulator, which is consistent with its location in the nucleus (5). It has been demonstrated that the ING1 gene has three exons and that four mRNA variants are transcribed from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 IG1) (6, 7). ING1 can possibly influence the above biological functions depending on which variant is expressed (1-4).

References :

1. Cheung, K.J., Jr. and Li, G. (2002) Int. J. Oncol. 21(6): 1361-1365. 2. Cheung, K.J., Jr. and Li, G. (2002) Exp. Cell Res. 279(2): 291-28. 3. Cheung, K.J., Jr. and Li, G. (2002) Int. J. Oncol. 20(6):1319-1322. 4. Cheung, K.J., Jr. and Li, G. (2001) Exp. Cell Res. 268(1): 1-6. 5. Garkavtsev, I. et al. (1996). Nat Genet. 14(4): 415-420. 6. Gunduz, M. et al. (2000). Cancer Res. 60(12): 3143-6. 7. Jager, D. et al. (1999). Cancer Res. 59: 6197-6204.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-145_p33-ING1_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-145_ING1_Antibody_WB_Mouse_brain-cell-lysates_1.png SPC-145_p33-ING1_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-p33 ING1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-145) Rabbit Anti-ING1 Antibody used in Western blot (WB) on Mouse brain cell lysates (SPC-145) Rabbit Anti-p33 ING1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-145)

more info or order :

StressMarq Biosciences product webpage