Rab4 Antibody | StressMarq Biosciences SPC-141D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Rab4 Antibody

catalog :

SPC-141D

quantity :

100 µl

price :

257.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SPC-141D

Product Name :

Rab4 Antibody

Description :

Rabbit Anti-Human Rab4 Polyclonal

Target :

Rab4

Conjugate :

Unconjugated

2021 List Price :

257.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Tags and Cell Markers Organelle Markers Neuroscience Cell Markers Neuron Markers Presynaptic Markers

Alternative Name(s) :

Oncogene Rab4 Antibody, Rab4A Antibody, Ras related protein Antibody

Size :

100 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

C-terminal peptide from human Rab4

Immunogen Species :

Human

Accession Number :

NP_004569.2

Swiss-Prot :

P20338

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:150); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~26kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-141D, RRID: AB_2177546

Certificate of Analysis :

A 1:1000 dilution of SPC-141 was sufficient for detection of Rab4 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Membrane Cytoplasm

Scientific Background :

Rab4 is a 25kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COHO-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins (3). Each Rab shows a characteristic sub-cellular distribution (4). In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin (5). Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol (6).

References :

1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7. 2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208. 3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412. 4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117. 5. Cormont M., et al. (1996) Mol Cell Biology. 16: 6879-6886. 6. Ayad N., Hull M., and Mellman I. (1997) EMBO 16: 4497-4507. 7. Van der Sluijs, P. et l. (1992) The EMBO Journal 11(12), 4379-4389.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-141_Rab4_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-141_Rab4_Antibody_WB_Human_HeLa-cell-lysates_1.png SPC-141_Rab4_Antibody_IHC_Mouse_backskin_5.png SPC-141_Rab4_Antibody_ICC-IF_Human_HaCaT-cells_6.png SPC-141_Rab4_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-Rab4 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-141) Rabbit Anti-Rab4 Antibody used in Western blot (WB) on Human Cervical cancer cell line (HeLa) lysate (SPC-141) Rabbit Anti-Rab4 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-141) Rabbit Anti-Rab4 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-141) Rabbit Anti-Rab4 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-141)

more info or order :

StressMarq Biosciences product webpage