Calnexin-NT Antibody | StressMarq Biosciences SPC-127B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Calnexin-NT Antibody

catalog :

SPC-127B

quantity :

200 µl

price :

336.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SPC-127B

Product Name :

Calnexin-NT Antibody

Description :

Rabbit Anti-Human Calnexin-NT Polyclonal

Target :

Calnexin

Conjugate :

Unconjugated

2021 List Price :

336.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Tags and Cell Markers Organelle Markers

Alternative Name(s) :

Calnexin antibody, CALX_HUMAN antibody, CANX antibody, CNX antibody, FLJ26570 antibody, Histocompatibility complex class I antigen binding protein p88 antibody, IP90 antibody, Major histocompatibility complex class I antigen-binding protein p88 antibody, P90 antibody

Size :

200 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

A 19 residue synthetic peptide based on dog calnexin and the peptide coupled to KLH

Immunogen Species :

Human

Accession Number :

NP_001003232.1

Swiss-Prot :

P24643

Applications :

WB IHC ICC/IF IP

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Ck Dg GP Hm Pg Mk Rb Sh Xe

Species Reactivity Full Name :

Human Mouse Rat Bovine Chicken Dog Guinea Pig (Cavia porcellus) Hamster Pig Monkey Rabbit Sheep African clawed frog (Xenopus laevis)

Antibody Dilution :

WB (1:5000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Rabbit Antiserum

Storage Buffer :

Rabbit Antiserum

Specificity :

Detects the N-terminal domain of Calnexin ~90kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-127B, RRID: AB_2068995

Certificate of Analysis :

A 1:5000 dilution of SPC-127 was sufficient for detection of Calnexin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Endoplasmic Reticulum Endoplasmic Reticulum Membrane Melanosome

Scientific Background :

Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their mono-glycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

References :

1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145): 387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3): 633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A. (1999) J. Cell Sci. 112: 4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1): 97-103. 7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18): 8452-6. 8. Raggo C., et al. (2002) Mol Cell Biol. 22: 5639-5649. 9. Rubio M.E., and Wenthold R.J. (1999) J Neurochem. 73: 942-948.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-127_Calnexin_Antibody_WB_Rat_tissue-mix_1.png SPC-127_Calnexin_Antibody_IHC_Mouse_colon-colitis_1.png
SPC-127_Calnexin_Antibody_IHC_Mouse_bac
kskin_1.png
SPC-127_Calnexin_Antibody_ICC-IF_Human_HeLa-cells_1.png SPC-127_Calnexin_Antibody_WB_Human_HeLa-cell-lysates_1.png

Image Alternative Text :

Rabbit Anti-Calnexin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-127) Rabbit Anti-Calnexin Antibody used in Western blot (WB) on Human Cervical cancer cell line (HeLa) lysate (SPC-127) Rabbit Anti-Calnexin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-127) Rabbit Anti-Calnexin Antibody used in Western blot (WB) on Rat tissue mix (SPC-127) Rabbit Anti-Calnexin Antibody used in Immunohistochemistry (IHC) on Mouse colon colitis (SPC-127)

more info or order :

StressMarq Biosciences product webpage