Alpha B Crystallin Antibody | StressMarq Biosciences SPC-126B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Alpha B Crystallin Antibody

catalog :

SPC-126B

quantity :

200 µl

price :

257.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SPC-126B

Product Name :

Alpha B Crystallin Antibody

Description :

Rabbit Anti-Human alpha B Crystallin Polyclonal

Target :

Alpha B Crystallin

Conjugate :

Unconjugated

2021 List Price :

257.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Neuroscience

Alternative Name(s) :

AACRYA Antibody, Alpha crystallin B chain Antibody, CRYA2 Antibody, CRYAB Antibody, CTPP2 Antibody, HSPB5 Antibody, NY REN 27 antigen Antibody

Size :

200 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH

Immunogen Species :

Human

Accession Number :

NP_001876.1

Swiss-Prot :

P02511

Applications :

WB IHC ICC/IF

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Ck

Species Reactivity Full Name :

Human Mouse Rat Bovine Chicken

Antibody Dilution :

WB (1:5000), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.

Purification :

Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~22kDa. Does not cross-react with αA-crystallin.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-126B, RRID: AB_2084306

Certificate of Analysis :

A 1:5000 dilution of SPC-126 was sufficient for detection of alpha B crystallin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).

References :

1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577. 9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-126_Alpha-B-Crystallin_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-126-Alpha-B-Crystallin-Antibody-WB-Human-A431-HCT116-HeLa-HepG2-HEK293-HUVEC-Jurkat-MCF7-PC3-and-T98G-cell-lysates-1.png SPC-126_Alpha-B-Crystallin_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SPC-126-Alpha-B-Crystallin-Antibody-WB-Rat-Brain-cell-lysates-1.png

Image Alternative Text :

Rabbit Anti-Alpha B Crystallin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-126) Rabbit Anti-Alpha B Crystallin Antibody used in Western blot (WB) on A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates (SPC-126) Rabbit Anti-Alpha B Crystallin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-126) Rabbit Anti-Alpha B Crystallin Antibody used in Western blot (WB) on Brain cell lysates (SPC-126)

more info or order :

StressMarq Biosciences product webpage