Calreticulin Antibody | StressMarq Biosciences SPC-122B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Calreticulin Antibody

catalog :

SPC-122B

quantity :

200 µl

price :

250.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

more info or order :

StressMarq Biosciences product webpage

citations: 1

Reference
Pujol F, Laketa V, Schmidt F, Mukenhirn M, MÃ¼ller B, Boulant S, et al. HIV-1 Vpu Antagonizes CD317/Tetherin by Adaptor Protein-1-Mediated Exclusion from Virus Assembly Sites. J Virol. 2016;90:6709-6723 pubmed publisher

product information

Catalog No :

SPC-122B

Product Name :

Calreticulin Antibody

Description :

Rabbit Anti-Human Calreticulin Polyclonal

Target :

Calreticulin

Conjugate :

Unconjugated

2021 List Price :

250.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Protein Trafficking Organelle Proteins Tags and Cell Markers Organelle Markers

Alternative Name(s) :

CALR Antibody, Calregulin Antibody, cC1qR Antibody, CRP55 Antibody, ERp60 Antibody, HSCBP Antibody, RO Antibody, SSA Antibody, grp60 Antibody

Size :

200 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH

Immunogen Species :

Human

Accession Number :

NP_004334.1

Swiss-Prot :

P27797

Applications :

WB IHC ICC/IF IP FCM

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Dg Ck GP Mk Pg Hm Rb Sh

Species Reactivity Full Name :

Human Mouse Rat Bovine Dog Chicken Guinea Pig (Cavia porcellus) Monkey Pig Hamster Rabbit Sheep

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~63kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-122B, RRID: AB_2069601

Certificate of Analysis :

A 1:1000 dilution of SPC-122 was sufficient for detection of Calreticulin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Endoplasmic Reticulum Endoplasmic Reticulum Lumen Cytoplasm Cell Surface Extracellular Matrix Sarcoplasmic Reticulum Sarcoplasmic Reticulum Lumen

Scientific Background :

Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).

References :

1. Johnson S., et al. (2001) Trends Cell Biol 11: 122-129. 2. Smith M.J., et al. (1989) EMBO J. 8: 3581-3586. 3. Ellgaard L., et al. (2001) Curr Opin Cell Biol. 13: 431-437. 4. Krause K.H., and Michalak M. (1997) Cell. 88: 439-443. 5. Nash P.D., et al. (1994) Mol Cell Biochem. 135: 71-78. 6. Heal R., and McGivan J. (1998) Biochem J. 329: 389-394. 7. Lucero H.A., et al. (1998) J Biol Chem. 273: 9857-9863. 8. Tanaka S., et al. (2000) J Biol Chem. 275: 10388-10393. 9. Yoon G.S., et al. (2000) Cancer Res. 60: 1117-1120. 10. Antoniou A.N., et al. (2002) Immunology 106: 182-189. 11. Wada I., et al. (1997) EMBO J. 16: 5420-5432. 12. Laguerre D.B., et al. (1998) J Vir. 72: 4940-4949.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-122_Calreticulin_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png
SPC-122_Calreticulin_Antibody_IHC_Mouse
_backskin_1.png
SPC-122_Calreticulin_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-122_Calreticulin_Antibody_WB_multiple-cell-lines_lysates_1.png

Image Alternative Text :

Rabbit Anti-Calreticulin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-122) Rabbit Anti-Calreticulin Antibody used in Western blot (WB) on multiple cell lines lysates (SPC-122) Rabbit Anti-Calreticulin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-122) Rabbit Anti-Calreticulin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-122)

more info or order :

StressMarq Biosciences product webpage