Ubiquitin Antibody | StressMarq Biosciences SPC-119B product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Ubiquitin Antibody

catalog :

SPC-119B

quantity :

200 µl

price :

306.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 1

Reference
Murakami A, Nagao K, Juni N, Hara Y, Umeda M. An N-terminal di-proline motif is essential for fatty acid-dependent degradation of Δ9-desaturase in Drosophila. J Biol Chem. 2017;292:19976-19986 pubmed publisher

product information

Catalog No :

SPC-119B

Product Name :

Ubiquitin Antibody

Description :

Rabbit Anti-Bovine Ubiquitin Polyclonal

Target :

Ubiquitin

Conjugate :

Unconjugated

2021 List Price :

306.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Ubiquitination Neuroscience Neurodegeneration Alzheimer's Disease

Alternative Name(s) :

Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody

Size :

200 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Native bovine Ubiquitin, conjugated to KLH

Immunogen Species :

Bovine

Accession Number :

NP_776558.1

Swiss-Prot :

P0CG53

Applications :

WB IHC ICC/IF IP

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Mk Hm Rb GP Pg Dg Sh Ck Xe Ys Dr Fs

Species Reactivity Full Name :

Human Mouse Rat Bovine Monkey Hamster Rabbit Guinea Pig (Cavia porcellus) Pig Dog Sheep Chicken African clawed frog (Xenopus laevis) Yeast Fruit Fly (Drosophila melanogaster) Fish Rainbow Trout (Oncorhynchus mykiss)

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~10kDa. It also recognizes ubiquinated proteins.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-119B, RRID: AB_2285248

Certificate of Analysis :

A 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cell Membrane Cytoplasm Nucleus

Scientific Background :

Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-119_Ubiquitin_Antibody_IHC_Human_colon-carcinoma_1.png SPC-119_Ubiquitin_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png
SPC-119_Ubiquitin_Antibody_IHC_Mouse_ba
ckskin_1.png
SPC-119_Ubiquitin_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-119_Ubiquitin_Antibody_WB_Human_HEK93-lysates_1.png

Image Alternative Text :

Rabbit Anti-Ubiquitin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-119) Rabbit Anti-Ubiquitin Antibody used in Western blot (WB) on Human Embryonic kidney epithelial cell line (HEK293) lysate (SPC-119) Rabbit Anti-Ubiquitin Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-119) Rabbit Anti-Ubiquitin Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-119) Rabbit Anti-Ubiquitin Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-119)

more info or order :

StressMarq Biosciences product webpage