SOD (Cu/Zn) Antibody | StressMarq Biosciences SPC-116D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

SOD (Cu/Zn) Antibody

catalog :

SPC-116D

quantity :

100 µg

price :

314.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Western blot analysis of Human Cell line lysates showing detection of SOD1 protein using Rabbit Anti-SOD1 Polyclonal Antibody (SPC-116). Load: 15 µgprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody (SPC-116) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

product information

Catalog No :

SPC-116D

Product Name :

SOD (Cu/Zn) Antibody

Description :

Rabbit Anti-Human SOD (Cu/Zn) Polyclonal

Target :

SOD1

Conjugate :

Unconjugated

2021 List Price :

314.00 USD

Currency :

USD

Research Area(s) :

Cancer Oxidative Stress Cell Signaling Protein Trafficking Chaperone Proteins Neuroscience Neurodegeneration ALS Disease

Alternative Name(s) :

Superoxide dismutase1 Antibody, ALS1 Antibody, IPOA Antibody, SOD1 Antibody, SOD2 Antibody, SODC Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Human Cu/Zn SOD

Immunogen Species :

Human

Accession Number :

NP_000445.1

Swiss-Prot :

P00441

Applications :

WB IHC IP ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Rt Ms Bv Mk Inv Co Dg Hm Pg Rb Sh Xe Mlsk Fs

Species Reactivity Full Name :

Human Rat Mouse Bovine Monkey Invertebrate Coral (Anthozoa) Dog Hamster Pig Rabbit Sheep African clawed frog (Xenopus laevis) Mollusk Mussel (Perna viridis) Fish (O. melastigma)

Antibody Dilution :

WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS pH7.0, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~23kDa (human) and ~19kDa (other species).

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-116D, RRID: AB_2239732

Certificate of Analysis :

0.2 µg/ml of SPC-116 was sufficient for detection of Cu/Zn SOD in 20 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Cellular Localization :

Cytoplasm

Scientific Background :

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

References :

1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-116_SOD1_Antibody_IHC_Human_Placenta_1.png SPC-116_SOD1_Antibody_WB_Human_Cell-line-lysates_1.png

Image Alternative Text :

Rabbit Anti-SOD1 Antibody used in Immunohistochemistry (IHC) on Human Placenta (SPC-116) Rabbit Anti-SOD1 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-116)

more info or order :

StressMarq Biosciences product webpage