antibody

PDI Antibody

SPC-114D

100 µl

304.00 USD

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat, dogs, bovine

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

PDI Antibody

Rabbit Anti-Rat PDI Polyclonal

PDI

Unconjugated

304.00 USD

USD

Cell Signaling Protein Trafficking Chaperone Proteins Tags and Cell Markers Organelle Markers

PDA2 Antibody, PDI Antibody, PDIA2 Antibody, PDIP Antibody, pancreatic protein disulfide isomerase Antibody

100 µl

Antibodies

Polyclonal

Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH

Rat

NP_001099245.2

P04785

WB IHC ICC/IF IP

Rabbit

Hu Ms Rt Bv Dg Hm GP Sh Pg Xe Mlsk

Human Mouse Rat Bovine Dog Hamster Guinea Pig (Cavia porcellus) Sheep Pig African clawed frog (Xenopus laevis) Mollusk Mussel (Perna viridis)

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Peptide Affinity Purified

PBS pH7.4, 50% glycerol, 0.09% sodium azide

1 mg/ml

Detects ~58kDa.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-114D, RRID: AB_2156444

A 1:1000 dilution of SPC-114 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Endoplasmic Reticulum Endoplasmic Reticulum Lumen

The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).

1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7. 2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5. 3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42. 4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95. 5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163. 6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819. 7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-114_PDI_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-114_PDI_Antibody_WB_Rat_tissue-mix_1.png SPC-114_PDI_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-114) Rabbit Anti-PDI Antibody used in Western blot (WB) on Rat tissue mix (SPC-114) Rabbit Anti-PDI Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-114)