BIM/BOD (IN) Antibody | StressMarq Biosciences SPC-113D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

BIM/BOD (IN) Antibody

catalog :

SPC-113D

quantity :

100 µg

price :

263.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

StressMarq Biosciences product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 4c	Thijssen R, Ter Burg J, van Bochove G, de Rooij M, Kuil A, Jansen M, et al. The pan phosphoinositide 3-kinase/mammalian target of rapamycin inhibitor SAR245409 (voxtalisib/XL765) blocks survival, adhesion and proliferation of primary chronic lymphocytic leukemia cells. Leukemia. 2016;30:337-45 pubmed publisher
	Peperzak V, Slinger E, Ter Burg J, Eldering E. Functional disparities among BCL-2 members in tonsillar and leukemic B-cell subsets assessed by BH3-mimetic profiling. Cell Death Differ. 2017;24:111-119 pubmed publisher

image

image 1 :

Western blot analysis of Human K562 (Left) and A549 (Right) whole cell lysates showing detection of BIM protein using Rabbit Anti-BIM Polyclonal Antibody (SPC-113). Primary Antibody: Rabbit Anti-BIM Polyclonal Antibody (SPC-113) at 1:1000.

product information

Catalog No :

SPC-113D

Product Name :

BIM/BOD (IN) Antibody

Description :

Rabbit Anti-Human BIM/BOD (IN) Polyclonal

Target :

BIM/BOD

Conjugate :

Unconjugated

2021 List Price :

263.00 USD

Currency :

USD

Research Area(s) :

Cancer Apoptosis Intracellular Neuroscience Neurodegeneration Alzheimer's Disease Immunology Innate Immunity Macrophage Cell Signaling Protein Phosphorylation Serine / Threonine Kinases MAPK Pathway Cancer Invasion/microenvironment Cancer Tumor Biomarkers Oncoproteins Cell Signaling Metabolism Metabolism processes

Alternative Name(s) :

BCL2 like protein 11 Antibody, Bcl2 interacting mediator of cell death Antibody, Bcl2 interacting protein Bim Antibody, BCL2 like 11 Antibody, BAM Antibody, BimEl Antibody, BimL Antibody, BOD Antibody, BIM beta 6 Antibody, BIM Beta7 Antibody, BIM alpha 3 Antibody, BIM alpha4 Antibody, BIM alpha5 Antibody, BIM alpha6 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Internal central amino acids of human Bim

Immunogen Species :

Human

Accession Number :

NP_619527.1

Swiss-Prot :

O43521

Applications :

WB IHC

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~23kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-113D, RRID: AB_2290432

Certificate of Analysis :

1 µg/ml of SPC-113 was sufficient for detection of Bim in 20 µg of K562 and A549 cell lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Mitochondrion Endomembrane System

Scientific Background :

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bim/BOD is a group of three splice variants, BimEL, BimL and BimS, with apparent molecular masses of ~23, 16, and 13 kDa, respectively. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1 ,2).

References :

1. O'Connor L., Strasser A., O'Reilly L.A., et al. (1998) EMBO J. 17: 384-395. 2. Hsu S.Y., Lin P., and Hsueh A.J. (1998) Mol. Endocrinol. 12: 1432-40.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-113_BIM_Antibody_IHC_Human_skin-cancer-cells_1.png SPC-113_BIM_Antibody_WB_Human_K562-Left-and-A549-Right-whole-cell-lysates_1.png

Image Alternative Text :

Rabbit Anti-BIM Antibody used in Immunohistochemistry (IHC) on Human skin cancer cells (SPC-113) Rabbit Anti-BIM Antibody used in Western blot (WB) on Human K562 (Left) and A549 (Right) whole cell lysates (SPC-113)

more info or order :

StressMarq Biosciences product webpage