product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
HSP27 Antibody
catalog :
SPC-106B
quantity :
200 µg
price :
250.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS
more info or order :
image
image 1 :
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-106). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp27 Polyclonal Antibody (SPC-106) at 1:250 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp27 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
product information
Catalog No :
SPC-106B
Product Name :
HSP27 Antibody
Description :
Rabbit Anti-Human HSP27 Polyclonal
Target :
HSP27
Conjugate :
Unconjugated
2021 List Price :
250.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Protein Trafficking Chaperone Proteins Cell Signaling Cytoskeleton Microfilaments Actin Actin Assembly Cell Signaling Cytoskeleton Microtubules Cancer Tumor Biomarkers Cardiovascular System Atherosclerosis Cardiovascular System Heart Contractility Cancer Heat Shock
Alternative Name(s) :
28kDa heat shock protein Antibody, CMT2F Antibody, HSP25 Antibody, HSP27 Antibody, HSP28 Antibody, HSPB1 Antibody, SRP27 Antibody
Size :
200 µg
Category :
Antibodies
Product Type :
Polyclonal
Immunogen :
Human HSP27, His tagged
Immunogen Species :
Human
Accession Number :
NP_001531.1
Swiss-Prot :
P04792
Applications :
WB ICC/IF FCM FACS IHC
Host Species :
Rabbit
Species Reactivity Abbreviation :
Hu Ms Shk Dg
Species Reactivity Full Name :
Human Mouse Shark Gummy Shark (Mustelus antarcticus) School Shark (Galeorhinus galeus) Dog
Antibody Dilution :
WB (1:2000), ICC/IF (1:250); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1.38 mg/ml
Specificity :
Detects ~27kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-106B, RRID: AB_11229711
Certificate of Analysis :
A 1:2000 dilution of SPC-106 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization :
Cytoplasm Nucleus
Scientific Background :
HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6). Looking for more information on HSP27? Visit our new HSP27 Scientific Resource Guide at http://www.HSP27.com.
References :
1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394(6693): 595-599.
2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56.
3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229.
4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70.
5. Sarto C., Binnz P.A., and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26.
6. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-106_Hsp27_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-106_HSP27_Antibody_IHC_Human_Skeletal-Muscle_1.png SPC-106_HSP27_Antibody_WB_Human_HeLa-cell-lysates_1.png SPC-106_Hsp27_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png
Image Alternative Text :
Rabbit Anti-Hsp27 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-106) Rabbit Anti-HSP27 Antibody used in Immunohistochemistry (IHC) on Skeletal Muscle (SPC-106) Rabbit Anti-HSP27 Antibody used in Western blot (WB) on Human Cervical cancer cell line (HeLa) lysate (SPC-106) Rabbit Anti-Hsp27 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-106)
more info or order :
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
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