HSP60 Antibody | StressMarq Biosciences SPC-105D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP60 Antibody

catalog :

SPC-105D

quantity :

100 µg

price :

273.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 2

Reference
Brockmann S, Freischmidt A, Oeckl P, Müller K, Ponna S, Helferich A, et al. CHCHD10 mutations p.R15L and p.G66V cause motoneuron disease by haploinsufficiency. Hum Mol Genet. 2018;27:706-715 pubmed publisher
Peksel B, Gombos I, Peter M, Tiszlavicz Á, Brameshuber M, Balogh G, et al. Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis. Sci Rep. 2017;7:15643 pubmed publisher

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody (SPC-105). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody (SPC-105) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

product information

Catalog No :

SPC-105D

Product Name :

HSP60 Antibody

Description :

Rabbit Anti-Human HSP60 Polyclonal

Target :

HSP60

Conjugate :

Unconjugated

2021 List Price :

273.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Tags and Cell Markers Organelle Markers

Alternative Name(s) :

CPN60 Antibody, GROEL Antibody, HLD4 Antibody, HSP 60 Antibody, HSP65 Antibody, HSPD1 Antibody, HuCHA60 Antibody, SPG 13 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Human HSP60 produced through recombinant DNA methods in E.coli

Immunogen Species :

Human

Accession Number :

NP_002147.2

Swiss-Prot :

P10809

Applications :

WB IHC IP ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bv Dg Ck Rb Hm

Species Reactivity Full Name :

Human Mouse Rat Bovine Dog Chicken Rabbit Hamster

Antibody Dilution :

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein A Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~60kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-105D, RRID: AB_10807230

Certificate of Analysis :

1 µg/ml of SPC-105 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Cellular Localization :

Mitochondrion Mitochondrion Matrix

Scientific Background :

In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).

References :

1. Hartl, F.U. (1996) Nature 381: 571-579. 2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366. 3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858. 4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297. 10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556. 11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929. 12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-105_Hsp60_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png
SPC-105_HSP60_Antibody_WB_Human_Dog_Mou
se_SKBR3_MDCK_and-MEF-cell-line-lysates
_1.png
SPC-105_Hsp60_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-105_HSP60_Antibody_IHC_Human_Adrenal_1.png

Image Alternative Text :

Rabbit Anti-Hsp60 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-105) Rabbit Anti-HSP60 Antibody used in Immunohistochemistry (IHC) on Adrenal (SPC-105) Rabbit Anti-HSP60 Antibody used in Western blot (WB) on Human, Dog, Mouse SKBR3, MDCK, and MEF cell line lysates (SPC-105) Rabbit Anti-Hsp60 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-105)

more info or order :

StressMarq Biosciences product webpage