HSP70 Antibody | StressMarq Biosciences SPC-103D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP70 Antibody

catalog :

SPC-103D

quantity :

100 µl

price :

297.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 6a	Watanabe Y, Tsujimura A, Taguchi K, Tanaka M. HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis. Autophagy. 2017;13:133-148 pubmed publisher
	Woodford M, Dunn D, Blanden A, Capriotti D, Loiselle D, Prodromou C, et al. The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding. Nat Commun. 2016;7:12037 pubmed publisher
	Codonho B, Costa S, Peloso E, Joazeiro P, Gadelha F, Giorgio S. HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics. Mem Inst Oswaldo Cruz. 2016;: pubmed publisher

product information

Catalog No :

SPC-103D

Product Name :

HSP70 Antibody

Description :

Rabbit Anti-Human HSP70 Polyclonal

Target :

HSP70

Conjugate :

Unconjugated

2021 List Price :

297.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers

Alternative Name(s) :

HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSP73 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody

Size :

100 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Full length protein HSP70

Immunogen Species :

Human

Accession Number :

NP_005336.3

Swiss-Prot :

P0DMV8/P0DMV9

Applications :

WB IHC ICC/IF IP ELISA

Host Species :

Rabbit

Species Reactivity Abbreviation :

Hu Ms Rt Bg Bv Dg Fs Cp GP Hm Mk Pg Sh Co Pl Shk L. am

Species Reactivity Full Name :

Human Mouse Rat Beluga (Delphinapterus leucas) Bovine Dog Fish Carp (Cypriniformes) Guinea Pig (Cavia porcellus) Hamster Monkey Pig Sheep Coral (Anthozoa) Plant Tomato Tobacco Shark Spiny Dogfish Shark (Squalus acanthias) Atlantic Hagfish (Myxine glutinosa) Leishmania amazonensis

Antibody Dilution :

WB (1:1000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Peptide Affinity Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects a ~70kDa. May cross-react with HSC70 at lower dilutions.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-103D, RRID: AB_2119380

Certificate of Analysis :

A 1:1000 dilution of SPC-103 was sufficient for detection of HSP70 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cellular Localization :

Cytoplasm

Scientific Background :

HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (1, 2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.

References :

1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Hung T.H., et al. (2001) Am J Pathol. 159: 1031-1043. 8. Locke M. (2000) Cell Stress & Chaperones 5: 45-51. 9. Ianaro A., et al. (2001) FEBS Lett. 508: 61-66. 10.Trentin G.A. et al. (2001) J Biol Chem. 276: 13087-13095.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-103_Hsp70_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-103_HSP70_Antibody_IHC_Human_colon-carcinoma_1.png SPC-103_HSP70_Antibody_WB_Human_Rat_brain-cell-lysates_1.png SPC-103_HSP70_Antibody_IHC_Mouse_backskin_3.png SPC-103_HSP70_Antibody_WB_Mouse_Pam212-cells_1.png SPC-103_Hsp70_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SPC-103_HSP70_Antibody_IHC_Mouse_Inflamed-colon_1.png

Image Alternative Text :

Rabbit Anti-Hsp70 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-103) Rabbit Anti-HSP70 Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-103) Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Human, Rat brain cell lysates (SPC-103) Rabbit Anti-HSP70 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-103) Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Mouse Pam212 cells (SPC-103) Rabbit Anti-Hsp70 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-103) Rabbit Anti-HSP70 Antibody used in Immunohistochemistry (IHC) on Mouse Inflamed colon (SPC-103)

more info or order :

StressMarq Biosciences product webpage