HSP40 Antibody | StressMarq Biosciences SPC-100D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP40 Antibody

catalog :

SPC-100D

quantity :

100 µl

price :

297.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

image

image 1 :

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp40 Polyclonal Antibody (SPC-100) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp40 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

product information

Catalog No :

SPC-100D

Product Name :

HSP40 Antibody

Description :

Rabbit Anti-Human HSP40 Polyclonal

Target :

HSP40

Conjugate :

Unconjugated

2021 List Price :

297.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins

Alternative Name(s) :

DnaJ (HSP40) homolog subfamily B member 1 Antibody, DNAJ1 Antibody, DNAJB1 Antibody, HDJ1 Antibody, HSPF1 Antibody

Size :

100 µl

Category :

Antibodies

Product Type :

Polyclonal

Immunogen :

Recombinant purified full-length human HSP40 (no tags)

Immunogen Species :

Human

Accession Number :

NP_006136.1

Swiss-Prot :

P25685

Applications :

WB ICC/IF IP

Host Species :

Rabbit

Species Reactivity Abbreviation :

Species Reactivity Full Name :

Human

Antibody Dilution :

WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Rabbit Antiserum

Storage Buffer :

Rabbit Antiserum

Specificity :

Detects ~40kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SPC-100D, RRID: AB_2261861

Certificate of Analysis :

0.5 µg/ml of SPC-100 was sufficient for detection of HSP40 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm Nucleus

Scientific Background :

DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (6), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (2).

References :

1. Melville, M. W. et al. (1997) PNAS USA, 94: 97-102. 2. Hattori, H., Liu, Y-C., Tohnai, I., Ueda, M., Kaneda, T., Kobayashi, T., Tanabe, K., and Ohtsuka, K. (1992) Cell Structure and Function 17: 77-86. 3. Ohtsuka, K. Masuda, A., Nakai, A., and Nagata, K. (1990) Biochem. Biophys. Res. Commun. 166: 642-647. 4. Bardwell, J.C.A., Tilly, K., Craig, E., King, J., Zylicz, M. and Georgopoulos, C. (1986) J. Biol. Chem. 261: 1782-1785. 5. Ohku, M., Tamura, F., Nishimura, S., and Uchida, H. (1986) J. Biol. Chem. 261: 1778-1781. 6. Ohtsuka, K. (1993) Biochem. Biophys. Res. Commun. 197: 235-240.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SPC-100_Hsp40_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-100_HSP40_Antibody_WB_Human_HeLa-cell-lysates_1.png SPC-100_Hsp40_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Image Alternative Text :

Rabbit Anti-Hsp40 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-100) Rabbit Anti-HSP40 Antibody used in Western blot (WB) on Human Cervical cancer cell line (HeLa) lysate (SPC-100) Rabbit Anti-Hsp40 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-100)

more info or order :

StressMarq Biosciences product webpage