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company name :
StressMarq Biosciences
product type :
antibody
product name :
TCP1-alpha Antibody
catalog :
SMC-479D
quantity :
100 µg
price :
278.00 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
91a
reactivity :
human, mouse, rat, dogs, bovine
application :
western blot, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-479D
Product Name :
TCP1-alpha Antibody
Description :
Rat Anti-Mouse TCP1-alpha Monoclonal IgG2a
Target :
TCP1 alpha
Conjugate :
Unconjugated
2021 List Price :
278.00 USD
Currency :
USD
Research Area(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking
Alternative Name(s) :
p63 Antibody, Tcp-1 Antibody, TCP1 alpha Antibody, Ccta Antibody, TRic Antibody, Tp63 Antibody, 21454 Antibody, c-cpn Antibody, CCT Antibody, Tcp1 Antibody, ccpn Antibody, AI528772 Antibody, Cct1 Antibody, Tcp 1 Antibody, c cpn Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
91a
Immunogen :
Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Immunogen Species :
Mouse
Accession Number :
NP_038714.2
Swiss-Prot :
P11983
Applications :
WB ICC/IF IP FCM
Host Species :
Rat
Isotype :
IgG2a
Species Reactivity Abbreviation :
Hu Ms Rt Bv Rb GP Hm Dg Pg Ys C.el Dr Mk
Species Reactivity Full Name :
Human Mouse Rat Bovine Rabbit Guinea Pig (Cavia porcellus) Hamster Dog Pig Yeast Yeast (Saccharomyces cerevisiae) Nematode (Caenorhabditis elegans) Fruit Fly (Drosophila melanogaster) Monkey
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.1% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-479D, RRID: AB_2702333
Certificate of Analysis :
1 µg/ml of SMC-479 was sufficient for detection of TCP1 alpha in 20 µg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-479_TCP1-alpha_Antibody_91a_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SMC-479_TCP1-alpha_Antibody_91a_WB_Human_A431-and-HEK293-cell-lysates_1.png SMC-479_TCP1-alpha_Antibody_91a_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.