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company name :
StressMarq Biosciences
product type :
antibody
product name :
ATF4 Antibody
catalog :
SMC-447D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S360A-24
reactivity :
human, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-447D
Product Name :
ATF4 Antibody
Description :
Mouse Anti-Human ATF4 Monoclonal IgG2a
Target :
ATF4
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Signaling Epigenetics and Nuclear Signaling
Alternative Name(s) :
Activating Transcription Factor 4 Antibody, ATF 4 Antibody, ATF4 protein Antibody, cAMP-dependent transcription factor ATF-4 Antibody, cAMP-responsive element-binding protein 2 Antibody, CREB 2 Antibody, CREB-2 Antibody, CREB2 Antibody, Cyclic AMP dependent transcription factor ATF 4 Antibody, Cyclic AMP response element binding protein 2 Antibody, Cyclic AMP response element binding protein 2 Antibody, Cyclic AMP-dependent transcription factor ATF-4 Antibody, Cyclic AMP-responsive element-binding protein 2 Antibody, DNA binding protein TAXREB67 Antibody, DNA binding protein TAXREB67 Antibody, DNA-binding protein TAXREB67 Antibody, Tax Responsive Enhancer Element B67 Antibody, Tax-responsive enhancer element-binding protein 67 Antibody, TAXREB67 Antibody, TXREB Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S360A-24
Immunogen :
Fusion protein amino acids 25-327 of human ATF4. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
Immunogen Species :
Human
Accession Number :
NP_001666.2
Swiss-Prot :
P18848
Applications :
WB IHC ICC/IF
Host Species :
Mouse
Isotype :
IgG2a
Species Reactivity Abbreviation :
Hu Rt
Species Reactivity Full Name :
Human Rat
Antibody Dilution :
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~60kDa. Does not cross-react with ATF5.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-447D, RRID: AB_2701793
Certificate of Analysis :
1 µg/ml of SMC-447 was sufficient for detection of ATF4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Cell Membrane Nucleus
Scientific Background :
Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-447-ATF4-Antibody-S360A-24-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SMC-447_ATF4_Antibody_S360A-24_WB_Rat_Brain_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.