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company name :
StressMarq Biosciences
product type :
antibody
product name :
Notch1 Antibody
catalog :
SMC-430D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S253-32
reactivity :
mouse, rat
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-430D
Product Name :
Notch1 Antibody
Description :
Mouse Anti-Mouse Notch1 Monoclonal IgG1
Target :
Notch1
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Signaling Epigenetics and Nuclear Signaling cardiovascular System Heart
Alternative Name(s) :
Neurogenic locus notch homolog protein 1 Antibody, hN1 Antibody, Translocation associated notch protein Antibody, TAN1 Antibody, TAN-1 Antibody, Motch A Antibody, mT14 Antibody, p300 Antibody, Motch Antibody, Mis6 Antibody, Neurogenic locus notch protein homolog Antibody, NICD Antibody, Notch 1 intracellular domain Antibody, Notch gene homolog 1 (Drosophila) Antibody, Notch homolog 1 Antibody, translocation-associated (Drosophila) Antibody, NOTCH Drosophila Antibody, homolog of 1 Antibody, Translocation associated notch homolog Antibody, Translocation associated notch protein TAN 1 Antibody, xotch Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S253-32
Immunogen :
Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
Immunogen Species :
Mouse
Accession Number :
NP_032740.3
Swiss-Prot :
Q01705
Applications :
WB ICC/IF
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Ms Rt
Species Reactivity Full Name :
Mouse Rat
Antibody Dilution :
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects >270kDa, ~120kDa and small fragments due to proteolysis. Does not cross-react with Notch 2 or Notch3.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-430D, RRID: AB_2701487
Certificate of Analysis :
1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cell Membrane Nucleus
Scientific Background :
Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
References :
1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719. 2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company. 3. Lindsell C.E., et al. (1995) Cell 80: 909-917. 4. Uyttendaele H., et al. (1996) Development 122: 2251-2259. 5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-430-Notch1-Antibody-S253-32-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SMC-430_Notch1_Antibody_S253-32_WB_Rat_Brain-Membrane_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.