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company name :
StressMarq Biosciences
product type :
antibody
product name :
Synaptotagmin-7 Antibody
catalog :
SMC-424D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S275-14
reactivity :
human, mouse
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-424D
Product Name :
Synaptotagmin-7 Antibody
Description :
Mouse Anti-Mouse Synaptotagmin-7 Monoclonal IgG2B
Target :
Synaptotagmin 7
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Markers Neuron Markers Presynaptic Markers
Alternative Name(s) :
IPCA-7 Antibody, PCANAP7 Antibody, Prostate cancer-associated protein 7 Antibody, SYT-7 Antibody, SYT7 Antibody, Synaptotagmin 7 Antibody, Synaptotagmin VII Antibody, SytVII Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S275-14
Immunogen :
Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
Immunogen Species :
Mouse
Accession Number :
NP_061271.1
Swiss-Prot :
Q9R0N7
Applications :
WB IHC ICC/IF
Host Species :
Mouse
Isotype :
IgG2B
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~45kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-424D, RRID: AB_2701379
Certificate of Analysis :
1 µg/ml of SMC-424 was sufficient for detection of Synaptotagmin-7 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasmic Vesicle
Scientific Background :
Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
References :
1. Schengrund C.L., et al. (2002) J Biol Chem. 277: 32815. 2. Reichardt L.F., et al. (1981) J Cell Biol. 91:257.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-424-Synaptotagmin-7-Antibody-S275-14-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SMC-424_Synaptotagmin-7_Antibody_S275-14_WB_Rat_brain-lysates_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.