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company name :
StressMarq Biosciences
product type :
antibody
product name :
Dicer Antibody
catalog :
SMC-416D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S167-7
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-416D
Product Name :
Dicer Antibody
Description :
Mouse Anti-Mouse Dicer Monoclonal IgG1
Target :
Dicer
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Epigenetics and Nuclear Signaling
Alternative Name(s) :
Dicer1 Antibody, DCR Antibody, DCR1 Antibody, DCR-1 Antibody, Double-strand-specific ribonuclease Antibody, Helicase with RNase motif Antibody, HERNA Antibody, Helicase MOI Antibody, KIAA0928 Antibody, Endoribonuclease Dicer Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S167-7
Immunogen :
Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
Immunogen Species :
Mouse
Accession Number :
NP_683750.2
Swiss-Prot :
Q8R418
Applications :
WB ICC/IF
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~215kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-416D, RRID: AB_2701235
Certificate of Analysis :
1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
References :
1. Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60. 2. Lee Y., et al. (2003) Nature 425: 415-9. 3. Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-416-Dicer-Antibody-S167-7-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SMC-416_Dicer_Antibody_S167-7_WB_Rat_Brain-Membrane_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.