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company name :
StressMarq Biosciences
product type :
antibody
product name :
GIT1 Antibody
catalog :
SMC-413D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S39B-8
reactivity :
human, mouse, rat
application :
western blot, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-413D
Product Name :
GIT1 Antibody
Description :
Mouse Anti-Rat GIT1 Monoclonal IgG1
Target :
GIT1
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Markers Neuron Markers Cytoplasmic Markers Cell Signaling Scaffold Proteins Post-translational Modifications Phosphorylation
Alternative Name(s) :
ARF GAP GIT1 Antibody, ARF GTPase-activating proteinAntibody, CAT1 Antibody, GRK-interacting protein 1 Antibody, ARF GTPase-activating protein GIT1 Antibody, Cool-associated and tyrosine-phosphorylated protein 1 Antibody, G protein-coupled receptor kinase-interactor 1 Antibody, GIT 1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S39B-8
Immunogen :
Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Immunogen Species :
Rat
Accession Number :
NP_114002.1
Swiss-Prot :
Q9Z272.1
Applications :
WB ICC/IF IP
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~90kDa. Does not cross-react with GIT2.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-413D, RRID: AB_2701181
Certificate of Analysis :
1 µg/ml of SMC-413 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm
Scientific Background :
G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT-1 and GIT-2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT-1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration (2). GIT-1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington disease (4). Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains (5). Htt interacts directly with GIT-1 causing enhanced htt proteolysis, indicating that GIT-1 distribution and function may contribute to Huntington disease pathology (4).
References :
1. Hoefen R.J. and Berk B.C. (2006) J. Cell Sci. 119: 146 1475. 2. Manabe R., et al. (2002) J. Cell Sci. 115: 1497-1510. 3. Zhang H., et al. (2003) J. Cell Biol. 161: 131-142. 4. Goehler H., et al. (2004) Mol. Cell 15: 853-865. 5. Mende-Mueller L.M., et al. (2001) J. Neurosci. 21: 1830-1837.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-413-GIT1-Antibody-S39B-8-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SMC-413_GIT1_Antibody_S39B-8_WB_Rat_brain-membrane-lysate_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.