product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
VGLUT3 Antibody
catalog :
SMC-397D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S34-34
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry
more info or order :
image
image 1 :
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGlut3 Monoclonal Antibody, Clone S34-34 (SMC-397). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGlut3 Monoclonal Antibody (SMC-397) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGlut3 Antibody. (D) Composite.
product information
Catalog No :
SMC-397D
Product Name :
VGLUT3 Antibody
Description :
Mouse Anti-Rat VGLUT3 Monoclonal IgG1
Target :
VGLUT3
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Markers Neuron Markers Presynaptic Markers Pumps/Transporters Neurotransmitter Transporters
Alternative Name(s) :
DFNA25 Antibody, Vesicular glutamate transporter 3 Antibody, deafness autosomal dominant 25 Antibody, SLC17A8 Antibody, Solute carrier family 17 member 8 Antibody, VGLUT 3 Antibody, Solute carrier family 17 (sodium dependent inorganic phosphate cotransporter) member 8 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S34-34
Immunogen :
Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGLUT3
Immunogen Species :
Rat
Accession Number :
NP_714947.1
Swiss-Prot :
Q7TSF2
Applications :
WB IHC ICC/IF
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~65kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-397D, RRID: AB_11229704
Certificate of Analysis :
1 µg/ml of SMC-397 was sufficient for detection of VGLUT3 in 20 µg of CV-1 fibroblast cells (lysate) transfected with VGLUT3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Membrane Cell Junction Synapse
Scientific Background :
Vesicular Glutamate Transporter 3 (VGLUT3) is a multipass membrane protein restricted to synaptic vesicles of glutamergic neurons. It is specifically expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord, and thalamus. Human VGLUT3 shares a 72% sequence homology with VLGUT2 and BNPI.
References :
1. Amilhon B., et al. (2010) J Neurosci. 30(6): 2198-2210.
2. Herzog E., et al. (2004) Neurosci. 123(4): 983-1002.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-397_VGlut3_Antibody_S34-34_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png
more info or order :
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.