product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
VGLUT1 Antibody
catalog :
SMC-394D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S28-9
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry
more info or order :
StressMarq Biosciences product webpage
citations: 1
Reference
Jewett K, Christian C, Bacos J, Lee K, Zhu J, Tsai N. Feedback modulation of neural network synchrony and seizure susceptibility by Mdm2-p53-Nedd4-2 signaling. Mol Brain. 2016;9:32 pubmed publisher
product information
Catalog No :
SMC-394D
Product Name :
VGLUT1 Antibody
Description :
Mouse Anti-Rat VGLUT1 Monoclonal IgG1
Target :
VGLUT1
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Markers Neuron Markers Presynaptic Markers Pumps/Transporters Neurotransmitter Transporters Cell Signaling
Alternative Name(s) :
BNPI Antibody, SLC17A1 Antibody, SLC17A1/VGLUT1 Antibody, Solute Carrier family 17 member 7 Antibody, Vesicular glutamate transporter 1 Antibody, VGLUT 1 Antibody, Brain-specific Na(+)-dependent inorganic phosphate cotransporter Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S28-9
Immunogen :
Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1
Immunogen Species :
Rat
Accession Number :
NP_446311.1
Swiss-Prot :
Q62634
Applications :
WB IHC
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~52kDa. No cross-reactivity against VGlut2.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-394D, RRID: AB_11229710
Certificate of Analysis :
1 µg/ml of SMC-394 was sufficient for detection of VGLut1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasmic Vesicle Secretory Vesicle Synaptic Vesicle Membrane Membrane Cell Junction Synapse
Scientific Background :
VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
References :
1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163. 2. Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-394_VGLUT1_Antibody_S28-9_IHC_Mouse_spinal cord_20X_1.png SMC-394_VGLUT1_Antibody_S28-9_WB_Rat_brain-membrane-lysate_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.