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company name :
StressMarq Biosciences
product type :
antibody
product name :
KCC2 Antibody
catalog :
SMC-392D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S1-12
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-392D
Product Name :
KCC2 Antibody
Description :
Mouse Anti-Rat KCC2 Monoclonal IgG2a
Target :
KCC2
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Pumps/Transporters Ion Pumps/Transporters
Alternative Name(s) :
Potassium Chloride Cotransporter Antibody, Potassium chloride transporter 5 Antibody, SLC12A5 Antibody, hKCC2 Antibody, S12A5 Antibody, Solute carrier family 12 member 5 Antibody, Electroneutral potassium-chloride cotransporter 2 Antibody, Furosemide-sensitive K-Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, rKCC2 Antibody, Neuronal K-Cl cotransporter Antibody, KIAA1176 Antibody, Electroneutral potassium chloride cotransporter 2 Antibody, Erythroid K Cl cotransporter 2 Antibody, Furosemide sensitive K Cl cotransporter Antibody, K-Cl cotransporter 2 Antibody, KCC 2 Antibody, Neuronal K Cl cotransporter Antibody, Solute carrier family 12 (potassium chloride transporter) member 5 Antibody, Solute carrier family 12 member 5 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
S1-12
Immunogen :
Fusion protein amino acids 932-1043 corresponding to rat KCC2
Immunogen Species :
Rat
Accession Number :
NP_599190
Swiss-Prot :
Q63633
Applications :
WB IHC ICC/IF IP
Host Species :
Mouse
Isotype :
IgG2a
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), IHC (1:300), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~140kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-392D, RRID: AB_11232615
Certificate of Analysis :
1 µg/ml of SMC-392 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Membrane
Scientific Background :
KCC2 is a member of the cation-chloride cotransporter gene family (1). It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain (2, 3). KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord (4). Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.
References :
1. Lee L.H., Walker J.A., Williams J.R., Goodier R.J., Payne J.A., Moss S.J. (2007) J Biol Chem. 282(41): 29777-29784. 2. Watanabe M., Wake H., Moorhouse A.J., Nabekura J. (2009) J Biol Chem. 284(41): 27980-27988. 3. Gulyas A.I., Sik A., Payne J.A., Kaila K., Freund T.F. (2001) Eur J Neurosci. 13(12): 2205-2217. 4. Vinay L., Jean-Xavier C. (2008) Brain Res Rev. 57(1): 103-110.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-392_KCC2_Antibody_S1-12_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SMC-392_KCC2_Antibody_S1-12_WB_Rat_brain-membrane-lysate_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.