antibody

HCN2 Antibody

SMC-305D

100 µg

300.00 USD

monoclonal

mouse

nonconjugated

S71

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

HCN2 Antibody

Mouse Anti-Rat HCN2 Monoclonal IgG1

HCN2

Unconjugated

300.00 USD

USD

Neuroscience Ion Channels Cyclic Nucleotide-Gated Ion Channels cardiovascular System Heart

BCNG2 Antibody, HAC1 Antibody, brain cyclic nucleotide gated channel 2 Antibody, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel Antibody

100 µg

Antibodies

Monoclonal

S71

Fusion protein amino acids 761-863 (cytoplasmic C-terminus) of rat HCN2

Rat

NP_446136.1

Q9JKA9

WB IHC ICC/IF IP AM

Mouse

IgG1

Hu Ms Rt

Human Mouse Rat

WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Protein G Purified

PBS pH7.4, 50% glycerol, 0.09% sodium azide

1 mg/ml

Detects ~95kDa. No cross-reactivity against HCN1.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SMC-305D, RRID: AB_2279443

1 µg/ml of SMC-305 was sufficient for detection of HCN2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Membrane

Hyperpolarization-activated cyclic nucleotide-gated ion channel 2 (HCN2) is an integral membrane protein that helps establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). Ion channels are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).

1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA. 2. www.iochannels.org 3. Zong X., et al. (2005) J Biol Chem. 280(40): 34224-34232

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SMC-305_HCN2_Antibody_S71-37_IHC_mouse_frozen-brain-section_1.png SMC-305_HCN2_Antibody_S71-37_WB_Rat_brain-membrane-lysate_1.png SMC-305_HCN2_Antibody_S71_WB_Mouse_Brain_2.png