product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
PARP Antibody
catalog :
SMC-259D
quantity :
100 µl
price :
260.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M3
reactivity :
human
application :
western blot
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SMC-259D image 1
Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293) showing detection of ~113 kDa PARP protein using Mouse Anti-PARP Monoclonal Antibody, Clone M3 (SMC-259). Lane 1: Human HeLa. Lane 2: Human 293T. Lane 3: Human Jurkat. Primary Antibody: Mouse Anti-PARP Monoclonal Antibody (SMC-259) at 1:2000. Predicted/Observed Size: ~113 kDa.
product information
Catalog No :
SMC-259D
Product Name :
PARP Antibody
Description :
Mouse Anti-Human PARP Monoclonal IgG1
Target :
PARP
Conjugate :
Unconjugated
2021 List Price :
260.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes ADP-ribosylation DNA/RNA DNA Damage and Repair Base excision repair DNA Damage Recognition
Alternative Name(s) :
ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody, ADP ribosyltransferase diphtheria toxin like 1 antibody, ADP ribosyltransferase NAD(+) antibody, ADPRT 1 antibody, ADPRT antibody, ADPRT1 antibody, ARTD1 antibody, msPARP antibody, NAD(+) ADP ribosyltransferase 1 antibody, NAD(+) ADP-ribosyltransferase 1 antibody, pADPRT 1 antibody, pADPRT1 antibody, PARP 1 antibody, PARP antibody, PARP-1 antibody, PARP1 antibody, PARP1_HUMAN antibody, Poly (ADP ribose) polymerase 1 antibody, poly (ADP ribose) polymerase family, member 1 antibody, Poly [ADP-ribose] polymerase 1 antibody, Poly(ADP ribose) polymerase antibody, poly(ADP ribose) synthetase antibody, poly(ADP ribosyl)transferase antibody, Poly[ADP ribose] synthetase 1 antibody, Poly[ADP-ribose] synthase 1 antibody, PPOL antibody
Size :
100 µl
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
M3
Immunogen :
A synthesized peptide derived from human PARP
Immunogen Species :
Human
Accession Number :
NP_001609.2
Swiss-Prot :
P09874
Applications :
WB
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:2000); optimal dilutions for assays should be determined by the user.
Purification :
Peptide Affinity Purified
Storage Buffer :
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
Specificity :
Antibody can detects endogenous PARP protein.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-259D, RRID: AB_2699808
Certificate of Analysis :
A 1:2000 dilution of SMC-259 was sufficient for detection of PARP in 10 µg of Human HeLa, 293T, Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus
Scientific Background :
Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes involving mainly DNA repair and programmed cell death. It is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
References :
1. Piskunova TS. (2008) Curr Gerontol Geriatr Res: 754190. 2. Espinoza LA. et al. (2007) Free Radic. Biol. Med. 42 (9): 1430–40. 3. Zerfaoui M. et al. (2008) Cell. Signal. 20 (1): 186–94.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-259_PARP_Antibody_WB_Human_HeLa_293
T_Jurkat_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.