product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
CD45 Antibody
catalog :
SMC-256D
quantity :
100 µl
price :
260.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
12A9
reactivity :
human
application :
western blot, immunohistochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SMC-256D image 1
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~147 kDa CD45 protein using Mouse Anti-CD45 Monoclonal Antibody, Clone 12A9 (SMC-256). Primary Antibody: Mouse Anti-CD45 Monoclonal Antibody (SMC-256) at 1:1000. Predicted/Observed Size: ~147 kDa.
product information
Catalog No :
SMC-256D
Product Name :
CD45 Antibody
Description :
Mouse Anti-Human CD45 Monoclonal IgG1
Target :
CD45
Conjugate :
Unconjugated
2021 List Price :
260.00 USD
Currency :
USD
Research Area(s) :
Immunology Adaptive Immunity B Cells B Cell CD Markers Immunology Adaptive Immunity T Cells T Cell CD Markers Immunology Cell Type Markers CD Markers Non-Lineage Markers Cancer Cancer Growth Inhibitors Tyrosine Kinase Inhibitors Neuroscience Cell Markers Glial Markers Microglia Markers Stem Cells Hematopoietic Progenitors HP Surface Molecules B Lymphocytic Lineage T Lymphocytic Lineage NK Cell Lineage Stem Cells Hematopoietic Progenitors Myeloid Dendritic Cell Lineage Lymphoid Monocytic Lineage Neutrophil Lineage Myeloid Mast Cell Lineage Immunology Adaptive Immunity Regulatory T Cells Angiogenesis
Alternative Name(s) :
B220 Antibody, CD 45 Antibody, CD45R Antibody, GP180 Antibody, LCA Antibody, Protein tyrosine phosphatase receptor type c polypeptide Antibody, PTPRC_HUMAN Antibody, T200 glycoprotein Antibody
Size :
100 µl
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
12A9
Immunogen :
Purified recombinant fragment of human CD45 expressed in E. Coli
Immunogen Species :
Human
Accession Number :
NP_563578.2
Swiss-Prot :
P08575
Applications :
WB IHC
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000); optimal dilutions for assays should be determined by the user.
Storage Buffer :
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
Specificity :
Detects endogenous CD45 proteins. (~147KD).
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-256D, RRID: AB_2699754
Certificate of Analysis :
A 1:1000 dilution of SMC-256 was sufficient for detection of CD45 in 10 µg of Human HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Membrane
Scientific Background :
CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, broadly expressed amount hematopoietic cells. There are kmultiple isoforms of CD45 distributed throughout the immune system, and they arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
References :
1. Barclay A., et al. (1997) The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge I.S., et al. (1993) Annu. Rev. Immunol. 12:85. 3. Kishihara K., et al. (1993) Cell. 74:143. 4. Pulido R., et al. (1988) J. Immunol. 140:3851.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-256_CD45_Antibody_WB_HeLa_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.