product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Histone H3 Antibody (tri Methyl Lys36)
catalog :
SMC-251D
quantity :
100 µl
price :
260.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Q12
application :
western blot
more info or order :
image
image 1 :
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~15 kDa Histone H3 protein using Mouse Anti-Histone H3 Monoclonal Antibody, Clone Q12 (SMC-251). Lane 1: 1:1,000 Dilution. Lane 2: 1:5,000 Dilution. Primary Antibody: Mouse Anti-Histone H3 Monoclonal Antibody (SMC-251). Predicted/Observed Size: ~15 kDa.
product information
Catalog No :
SMC-251D
Product Name :
Histone H3 Antibody (tri Methyl Lys36)
Description :
Mouse Anti-Human Histone H3 (tri Methyl Lys36) Monoclonal IgG1
Target :
Histone H3
Conjugate :
Unconjugated
2021 List Price :
260.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Epigenetics and Nuclear Signaling Histones H3 H3 Acetylation
Alternative Name(s) :
H3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
Size :
100 µl
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
Q12
Immunogen :
Prepared by immunizing synthetic peptide coupled to KLH.
Immunogen Species :
Human
Swiss-Prot :
P68431/Q71DI3/P84243
Applications :
WB
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:5000); optimal dilutions for assays should be determined by the user.
Storage Buffer :
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
Specificity :
Detects endogenous Histone H3(tri methyl K36) protein.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-251D, RRID: AB_2699664
Certificate of Analysis :
A 1:5000 dilution of SMC-251 was sufficient for detection of Histone H3 in 10 µg of Human HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus Chromosome
Scientific Background :
Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
References :
1. Wu R., et al. (1981) Cell. 27:321.
2. Strahl B., et al. (2000) Nature. 403:41.
3. Bauer U., et al. (2002) EMBO Rep. 3:39.
4. Frank D., et al. (2003) Gene. 312:135.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-251_HistoneH3_Antibody_WB_HeLa_1.png
more info or order :
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.