HSP70 Antibody | StressMarq Biosciences SMC-249D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

HSP70 Antibody

catalog :

SMC-249D

quantity :

100 µg

price :

357.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1H11

reactivity :

human, mouse, rat, bovine

application :

western blot, immunocytochemistry, flow cytometry, FACS

more info or order :

StressMarq Biosciences product webpage

citations: 1

Reference
Barreca M, Spinello W, Cavalieri V, Turturici G, Sconzo G, Kaur P, et al. Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway. J Cell Physiol. 2017;232:1845-1861 pubmed publisher

product information

Catalog No :

SMC-249D

Product Name :

HSP70 Antibody

Description :

Mouse Anti-Human Cell Membrane HSP70 Monoclonal IgG1

Target :

HSP70

Conjugate :

Unconjugated

2021 List Price :

357.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers Heat Shock

Alternative Name(s) :

HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

1H11

Immunogen :

Human native HSP70 protein

Immunogen Species :

Human

Accession Number :

NP_005336.3

Swiss-Prot :

P0DMV8/P0DMV9

Applications :

WB ICC/IF FCM FACS

Host Species :

Mouse

Isotype :

IgG1

Species Reactivity Abbreviation :

Hu Ms Rt Bv Pg

Species Reactivity Full Name :

Human Mouse Rat Bovine Pig

Antibody Dilution :

WB (1:1000), ICC/IF (1:100), FACS (1:250); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide

Concentration :

0.5 mg/ml

Specificity :

Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa band in Western Blot.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-249D, RRID: AB_2699628

Certificate of Analysis :

A 1:250 dilution of SMC-249 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.

Cellular Localization :

Cell membrane

Tissue Specificity :

Induced by heat shock.

Scientific Background :

HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6). Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9). Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.

References :

1. Gribaldo, S. et al. (1999) J. Bacteriol. 181, 434-443. 2. Sharma, D. & Masison, D.C. (2009) Protein Pept. Lett. 16, 571-581. 3. Sharma, D. et al. (2009) PLoS. ONE.4, e6644. 4. Kampinga, H.H. & Craig, E.A. (2010) Nat. Rev. Mol. Cell Biol. 11, 579-592. 5. Nickel, W. & Seedorf, M. (2008) Annu. Rev. Cell Dev. Biol. 24, 287-308. 6. Multhoff, G. & Hightower, L.E. (1996) Cell Stress. Chaperones. 1, 167-176. 7. De Maio, A. (2011) Cell Stress. Chaperones. 16, 235-249. 8. Horvath, I. & Vigh, L. (2010) Nature. 463, 436-438. 9. Horvath, I., Multhoff, G., Sonnleitner, A., & Vigh, L. (2008) Biochim. Biophys. Acta 1778, 1653-1664.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-249-HSP70-Antibody-1H11-ICC-IF-Human-HCT116-cells-1.png SMC-249-HSP70-Antibody-1H11-FACS-Human-Jurkat-E61-1.png

more info or order :

StressMarq Biosciences product webpage