product summary
Loading...
company name :
StressMarq Biosciences
product type :
antibody
product name :
Ubiquitin Antibody
catalog :
SMC-214D
quantity :
100 µg
price :
158.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
FK2
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SMC-214D image 1
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK2 (SMC-214). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody (SMC-214) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
product information
Catalog No :
SMC-214D
Product Name :
Ubiquitin Antibody
Description :
Mouse Anti-Human Ubiquitin Monoclonal IgG1
Target :
Ubiquitin
Conjugate :
Unconjugated
2021 List Price :
158.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Post-translational Modifications Ubiquitination Neuroscience Neurodegeneration Alzheimer's Disease
Alternative Name(s) :
Polyubiquitin B Antibody, RPS27A Antibody, UBA52 Antibody, UBB Antibody, UBC Antibody, ubiquitin B Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
FK2
Immunogen :
Ubiquitin conjugated lysozyme
Immunogen Species :
Human
Accession Number :
BAC56955.1
Swiss-Prot :
P0CG47
Applications :
WB IHC ICC/IF IP ELISA
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
ALL
Species Reactivity Full Name :
Species Independent
Antibody Dilution :
WB (1:10000); optimal dilutions for assays should be determined by the user.
Purification :
Protein A Purified
Storage Buffer :
10mM phosphate buffer, 0.15M NaCl pH7.4, 0.1% sodium azide
Concentration :
10 mg/ml
Specificity :
Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-214D, RRID: AB_2699358
Certificate of Analysis :
A 1:5000 dilution of SMC-214 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Nucleus
Scientific Background :
Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
References :
1. Chen J., & Chen Z. (2013). Curr Opin Immunol. (1): 4-12. 2. Shaid S., Brandts C., Serve H., & Dikic I. (2013). Cell Death Differ. 20(1): 21-30.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-214-Ubiquitin-Antibody-FK2-ICC-IF-Mouse-Fibroblast-cell-line-NIH-3T3-60X-Composite-1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.