GRP78 Antibody | StressMarq Biosciences SMC-211D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

GRP78 Antibody

catalog :

SMC-211D

quantity :

100 µg

price :

291.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3G12-1G11

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SMC-211D

Product Name :

GRP78 Antibody

Description :

Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa

Target :

GRP78

Conjugate :

Unconjugated

2021 List Price :

291.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Organelle Markers

Alternative Name(s) :

78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regulated Protein 78kDa Antibody, GRP 78 Antibody, GRP-78 Antibody, GRP78_HUMAN Antibody, Heat shock 70 kDa protein 5 Antibody, Heat Shock 70kDa Protein 5 Antibody, HSCe70 Antibody, HSPA 5 Antibody, HSPA5 Antibody, Immunoglobulin Heavy Chain Binding Protein Antibody, Immunoglobulin heavy chain-binding protein Antibody, mBiP Antibody, MIF2 Antibody, Sez7 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

3G12-1G11

Immunogen :

Full-length recombinant rat GRP78

Immunogen Species :

Rat

Accession Number :

NP_037215.1

Swiss-Prot :

P06761

Applications :

WB ICC/IF ELISA

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Hu Ms Rt

Species Reactivity Full Name :

Human Mouse Rat

Antibody Dilution :

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~78kDa.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-211D, RRID: AB_2699304

Certificate of Analysis :

1 µg/ml of SMC-211 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Endoplasmic Reticulum Endoplasmic Reticulum Lumen Melanosome

Scientific Background :

GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer's patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

References :

1. Cho, S. et al. (2007). Mol Cell Biol 27(1): 368-83. 2. Yang, Y. et al. (1998) J Biol Chem 273: 25552-25555. 3. Luo, S. et al (2006) 26 (15): 5688-97. 4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314. 5. Koomagi, R. et al. (1999) Anticancer Res. 19: 4333-4336. 6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949. 7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-211-GRP78-Antibody-3G12-1G11-ICC-IF-Mouse-Fibroblast-cell-line-NIH-3T3-60X-Composite-1.png SMC-211_GRP78_Antibody_3G12-1G11_WB_Human-Mouse-Rat_HEK-293-NIH3T3-and-Rat-Brain-cell-lysates_1.png

more info or order :

StressMarq Biosciences product webpage