product summary
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company name :
StressMarq Biosciences
product type :
antibody
product name :
Alginate Antibody
catalog :
SMC-209D
quantity :
100 µg
price :
321.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
3G4-1F5
application :
western blot, immunohistochemistry
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SMC-209D image 1
Western Blot analysis of ALL BSA-Alginate Conjugate showing detection of ~250 kDa Alginate protein using Mouse Anti-Alginate Monoclonal Antibody, Clone 3G4-1F5 (SMC-209). Lane 1: MW ladder. Lane 2: 0.625ug BSA:Alginate. Load: 0.625 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Alginate Monoclonal Antibody (SMC-209) at 1:500 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:100 for 1 hour at RT. Color Development: TMB solution for 2 min at RT. Predicted/Observed Size: ~250 kDa.
product information
Catalog No :
SMC-209D
Product Name :
Alginate Antibody
Description :
Mouse Anti- Alginate Monoclonal IgG1 Kappa
Target :
Alginate
Conjugate :
Unconjugated
2021 List Price :
321.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Post-translational Modifications
Alternative Name(s) :
Alginic Acid Antibody, Algin Antibody, Sodium Alginate Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
3G4-1F5
Immunogen :
Sodium Alginate conjugated to KLH
Applications :
WB IHC
Host Species :
Mouse
Isotype :
IgG1 Kappa
Species Reactivity Abbreviation :
ALL
Species Reactivity Full Name :
Species Independent
Antibody Dilution :
WB (1:500); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Binds selectively to a BSA-conjugated alginate, but not to unconjugated BSA.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-209D, RRID: AB_2699268
Certificate of Analysis :
1 µg/ml of SMC-209 was sufficient for detection of 150 ng of alginate-conjugated BSA by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Scientific Background :
Sodium alginate is used in biological experiments for the immobilization of cells and encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+. Studies suggest that preparation of alginate microspheres will sustain protein delivery within tissue scaffolds (1), as well as in many other cell types (2).
References :
1. Zhai P., Chen X.B., and Schreyer D.J. (2013) Biofabrication. 5(1): 015009. 2. Selimoglu S.M., Ayyildiz-Tamis D., Gurhan I.D., and Elibol M. (2012) J Biosci Bioeng. 113(2):233-238.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-209_Alginate_Antibody_3G4-1F5_WB_ALL_BSA-Alginate-Conjugate_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.