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company name :
StressMarq Biosciences
product type :
antibody
product name :
EndoPDI Antibody
catalog :
SMC-204D
quantity :
100 µg
price :
300.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2E7/7
reactivity :
human
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
StressMarq Biosciences product webpage
image
image 1 :
StressMarq Biosciences SMC-204D image 1
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EndoPDI Monoclonal Antibody, Clone 2E7/7 (SMC-204). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EndoPDI Monoclonal Antibody (SMC-204) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.
product information
Catalog No :
SMC-204D
Product Name :
EndoPDI Antibody
Description :
Mouse Anti-Human EndoPDI Monoclonal IgG2a
Target :
PDI
Conjugate :
Unconjugated
2021 List Price :
300.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Protein Trafficking Chaperone Proteins Organelle Markers
Alternative Name(s) :
Thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide isomeras Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
2E7/7
Immunogen :
Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
Immunogen Species :
Human
Accession Number :
NP_001139021
Swiss-Prot :
Q8NBS9
Applications :
WB IHC ICC/IF IP ELISA
Host Species :
Mouse
Isotype :
IgG2a
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:200); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~48kDa.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-204D, RRID: AB_2699214
Certificate of Analysis :
1 µg/ml of SMC-204 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Endoplasmic Reticulum Endoplasmic Reticulum Lumen
Scientific Background :
Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
References :
1. Sullivan D.C., et al. (2003) J Biol Chem. 278 (47) 47079-47088.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-204-EndoPDI-Antibody-2E7-7-ICC-IF-Human-Cervical-Cancer-cell-line-HeLa-60X-Composite-1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.