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company name :
StressMarq Biosciences
product type :
antibody
product name :
CENP-A Antibody
catalog :
SMC-202D
quantity :
100 µg
price :
278.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
5A7-2E11
reactivity :
human
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-202D
Product Name :
CENP-A Antibody
Description :
Mouse Anti-Human CENP-A Monoclonal IgG1
Target :
CENPA
Conjugate :
Unconjugated
2021 List Price :
278.00 USD
Currency :
USD
Research Area(s) :
Cell Signaling Epigenetics and Nuclear Signaling
Alternative Name(s) :
CENP A Antibody, cenpa Antibody, Centromere auto antigen A Antibody, Histone H3 like centromeric protein A Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
5A7-2E11
Immunogen :
Synthetic peptide corresponding to a portion of human CENP-A
Immunogen Species :
Human
Accession Number :
NP_001035891.1
Swiss-Prot :
P49450
Applications :
WB ICC/IF
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu
Species Reactivity Full Name :
Human
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~18kDa. Recognizes Human CENP-A.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-202D, RRID: AB_2699178
Certificate of Analysis :
1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus Chromosome Centromere Kinetochore
Scientific Background :
A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
References :
1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318. 2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188. 3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223. 4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-202-CENP-A-Antibody-5A7-2E11-ICC-IF-Human-Colon-cancer-cell-line-HT-29-60X-Composite-1.png SMC-202_CENP-A_Antibody_5A7-2E11_WB_Human_U2OS-cell-lysate_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.