DNMT1 Antibody | StressMarq Biosciences SMC-200D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

DNMT1 Antibody

catalog :

SMC-200D

quantity :

100 µg

price :

300.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

60B1220.1

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SMC-200D

Product Name :

DNMT1 Antibody

Description :

Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa

Target :

DNMT1

Conjugate :

Unconjugated

2021 List Price :

300.00 USD

Currency :

USD

Research Area(s) :

Cell Signaling Post-translational Modifications Organelle Markers

Alternative Name(s) :

DNA Mtase Antibody, DNMT Antibody, MCMT Antibody, DNA methyltransferase 1 Antibody, AIM Antibody, CXXC9 Antibody, DNMT Antibody, DNA (cytosine-5)-methyltransferase 1 Antibody, CXXC-type zinc finger protein 9 Antibody, DNA methyltransferase HsaI Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

60B1220.1

Immunogen :

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

Immunogen Species :

Human

Accession Number :

NP_001370

Swiss-Prot :

P26358

Applications :

WB IHC IP ChIP

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Hu Ms Fs Zf

Species Reactivity Full Name :

Human Mouse Fish Zebrafish (Danio rerio)

Antibody Dilution :

WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~180kDa. It will cross-react with mouse DNMT1.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-200D, RRID: AB_2293057

Certificate of Analysis :

2 µg/ml of SMC-200 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Nucleus

Scientific Background :

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

References :

1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-200_DNMT1_Antibody_60B1220-1_IHC_Human_colon-carcinoma_40x_1.png SMC-200_DNMT1_Antibody_60B1220-1_WB_Human_H1299-cell-lysate_2.png SMC-200_DNMT1_Antibody_60B1220-1_IHC_Mouse_medullar-kidney-tissue_1.png

more info or order :

StressMarq Biosciences product webpage