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company name :
StressMarq Biosciences
product type :
antibody
product name :
MDC1 Antibody
catalog :
SMC-197D
quantity :
100 µg
price :
278.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P2B11
reactivity :
human, mouse, bovine
application :
western blot, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-197D
Product Name :
MDC1 Antibody
Description :
Mouse Anti-Mouse MDC1 Monoclonal IgG1
Target :
MDC1
Conjugate :
Unconjugated
2021 List Price :
278.00 USD
Currency :
USD
Research Area(s) :
Cancer Oxidative Stress Cell Signaling Epigenetics and Nuclear Signaling
Alternative Name(s) :
Nuclear factor with BRCT domains1 Antibody, mediator of DNA damage checkpoint 1 Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
P2B11
Immunogen :
GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Immunogen Species :
Mouse
Accession Number :
NP_001010833.2
Swiss-Prot :
Q5PSV9
Applications :
WB ICC/IF
Host Species :
Mouse
Isotype :
IgG1
Species Reactivity Abbreviation :
Hu Ms Bv Cz
Species Reactivity Full Name :
Human Mouse Bovine Chimpanzee
Antibody Dilution :
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~184kDa. This antibody recognizes MDC1 at and around the N-terminus.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-197D, RRID: AB_10984183
Certificate of Analysis :
0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization :
Nucleus
Scientific Background :
MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.
References :
1. Lou Z., et al. (2004) J Biol Chem. 279(45): 46359-46362. 2. Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199. 3. Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814. 4. Wilson K.A., et al. (2011) Mol Cancer Res. 9(6): 766-781.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-197-MDC1-Antibody-P2B11-ICC-IF-Mouse-Fibroblast-cell-line-NIH-3T3-60X-Composite-1.png SMC-197-MDC1-Antibody-P2B11-WB-Human-293Trap-cell-lysates-1.png SMC-197-MDC1-Antibody-P2B11-WB-Mouse-Cortex-and-Cerebellum-1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.