Tsp23 Antibody | StressMarq Biosciences SMC-194D product information

product summary

company name :

StressMarq Biosciences

product type :

antibody

product name :

Tsp23 Antibody

catalog :

SMC-194D

quantity :

100 µg

price :

278.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Tsp232A

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

StressMarq Biosciences product webpage

product information

Catalog No :

SMC-194D

Product Name :

Tsp23 Antibody

Description :

Mouse Anti-Human Tsp23 Monoclonal IgG1 Kappa

Target :

TSP23

Conjugate :

Unconjugated

2021 List Price :

278.00 USD

Currency :

USD

Research Area(s) :

Cancer Heat Shock Cell Signaling Epigenetics and Nuclear Signaling

Alternative Name(s) :

Transcript similar p23 Antibody, AARSD1 Antibody, AASD1_HUMAN Antibody, Alanyl-tRNA editing protein Aarsd1 Antibody, Alanyl-tRNA synthetase domain containing 1 Antibody, Alanyl-tRNA synthetase domain-containing protein 1 Antibody, MGC2744 Antibody, OTTHUMP00000204017 Antibody, OTTHUMP00000204019 Antibody, OTTHUMP00000204020 Antibody

Size :

100 µg

Category :

Antibodies

Product Type :

Monoclonal

Clone Number :

Tsp232A

Immunogen :

Recombinant tsp23

Immunogen Species :

Human

Accession Number :

NP_001136126.1

Swiss-Prot :

B9A003

Applications :

WB IHC AM

Host Species :

Mouse

Isotype :

IgG1 Kappa

Species Reactivity Abbreviation :

Hu Ms

Species Reactivity Full Name :

Human Mouse

Antibody Dilution :

WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.

Purification :

Protein G Purified

Storage Buffer :

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Concentration :

1 mg/ml

Specificity :

Detects ~19kDa. Detects Tsp23, no cross-reactivity to p23.

Storage Temperature :

-20ºC

Shipping Temperature :

Blue Ice or 4ºC

Cite this Product :

StressMarq Biosciences Cat# SMC-194D, RRID: AB_10805544

Certificate of Analysis :

1 µg/ml of SMC-194 was sufficient for detection of tsp23 in 20 µg of transiently transfected Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Cellular Localization :

Cytoplasm

Scientific Background :

p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology. Specifically TSP23 (transcript similar p23) displays 44% and 17% amino acid identity with p23 and Sba1p respectively (10).

References :

1. Johnson J.L., Beito T. G., Krco C.J., Toft D.O. (1994) Mol Cell Biol 14: 1956-63. 2. Weikl T., Abelmann K., Buchner J. (1999) J Mol Biol 293: 685-91. 3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A., Toft D.O. (2000) J Biol Chem 275: 23045-52. 4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-50. 5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70. 6. Holt S.E., et al. (1999) Genes Dev 13: 817-26. 7. Hu J., Toft D., Anselmo D., Wang X. (2002) J Virol 76: 269-79. 8. Felts, S.J., Toft D.O. (2003) Cell Stress Chaperones.8: 108-13. 9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H., Vandekerckhove J. & Doskeland S.O. (2004) Leukemia 10. Freeman B.C., Felts S.J., Toft D.O., Yamamato K.R. (2000) Genes Dev. 14(4): 433-434.

Field of Use :

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Image Filenames :

SMC-194_Tsp23_Antibody_TSp232A_WB_Human_Heat-Shocked-HeLa-cell-lysates_1.png
SMC-194_Tsp23_Antibody_TSp232A_IHC_Mous
e_Heart-Skeletal-Muscle_1.png

more info or order :

StressMarq Biosciences product webpage