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company name :
StressMarq Biosciences
product type :
antibody
product name :
Piccolo Antibody
catalog :
SMC-188D
quantity :
100 µg
price :
294.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
6H9-B6
reactivity :
human, mouse, rat
application :
western blot, ELISA, immunocytochemistry
more info or order :
StressMarq Biosciences product webpage
product information
Catalog No :
SMC-188D
Product Name :
Piccolo Antibody
Description :
Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa
Target :
Piccolo
Conjugate :
Unconjugated
2021 List Price :
294.00 USD
Currency :
USD
Research Area(s) :
Neuroscience Cell Markers Neuron Markers Presynaptic Markers
Alternative Name(s) :
ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody
Size :
100 µg
Category :
Antibodies
Product Type :
Monoclonal
Clone Number :
6H9-B6
Immunogen :
Full length recombinant protein (GenBank ID: BC001304.1)
Immunogen Species :
Mouse
Accession Number :
NP_001104266.1
Swiss-Prot :
Q9QYX7
Applications :
WB ICC/IF ELISA
Host Species :
Mouse
Isotype :
IgG2b Kappa
Species Reactivity Abbreviation :
Hu Ms Rt
Species Reactivity Full Name :
Human Mouse Rat
Antibody Dilution :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification :
Protein G Purified
Storage Buffer :
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration :
1 mg/ml
Specificity :
Detects ~550kDa. No cross-reactivity to Bassoon.
Storage Temperature :
-20ºC
Shipping Temperature :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-188D, RRID: AB_10687596
Certificate of Analysis :
1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
Cellular Localization :
Cell Junction Synapse
Scientific Background :
Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
References :
1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-188-Piccolo-Antibody-6H9-B6-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SMC-188_Piccolo_Antibody_6H9-B6_WB_Rat-and-mouse_brain-lysates_1.png
more info or order :
StressMarq Biosciences product webpage
company information
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
headquarters: canada
StressMarq Biosciences Inc. is a bioreagents company producing high-quality antibodies, antibody conjugates, proteins, assay kits, and small molecules for the life sciences.
With over 17,000 products, we offer a wide range of products for scientists in cancer, neuroscience, epigenetics, cell signalling, and cellular stress research areas.
Based in Victoria, BC, with a small but dedicated group of scientists, StressMarq provides highly-validated products that are sold with our quality guarantee, and supported by our years of scientific expertise. Our products are available in over 50 countries through our extensive distributor network.
StressMarq draws on scientific excellence from around the globe. We strive to partner with academic or for-profit institutions through licensing agreements to bring cutting-edge research tools to the scientific community.